A bacterial guanine nucleotide exchange factor activates ARF on Legionella phagosomes
A bacterial guanine nucleotide exchange factor activates ARF on Legionella phagosomes
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DOI：
10.1126/science.1067025
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发表时间：
2002-01-25
期刊：
影响因子：
56.9
通讯作者：
Roy, CR
中科院分区：
文献类型：
--
作者：
Nagai, H;Kagan, JC;Roy, CR
细胞内病原体嗜肺军团菌破坏真核宿主细胞中的囊泡运输，以产生支持复制的空泡。dot/icm基因编码一种蛋白质分泌装置，pneumophila需要这种空泡的生物发生。这里我们证明L.嗜肺菌产生称为RalF的蛋白质，其作为鸟苷三磷酸酶（GTP酶）的ADP核糖基化因子（ARF）家族的交换因子起作用。RalF蛋白是ARF在含L.嗜肺菌RalF蛋白通过吞噬体膜的转运是一个点/公里依赖性过程。因此，RalF是Dot/Icm分泌装置的底物。
The intracellular pathogen Legionella pneumophila subverts vesicle traffic in eukaryotic host cells to create a vacuole that supports replication. The dot/icm genes encode a protein secretion apparatus that L. pneumophila require for biogenesis of this vacuole. Here we show that L. pneumophila produce a protein called RalF that functions as an exchange factor for the ADP ribosylation factor (ARF) family of guanosine triphosphatases (GTPases). The RalF protein is required for the localization of ARF on phagosomes containing L. pneumophila. Translocation of RalF protein through the phagosomal membrane is a dot/km-dependent process. Thus, RalF is a substrate of the Dot/Icm secretion apparatus.