Controlled release of bovine serum albumin from hydroxyapatite microspheres for protein delivery system
Controlled release of bovine serum albumin from hydroxyapatite microspheres for protein delivery system
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DOI：
10.1016/j.mseb.2007.09.006
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发表时间：
2008-02-25
影响因子：
3.6
通讯作者：
Fukumori, Yoshinobu
中科院分区：
文献类型：
--
作者：
Boonsongrit, Yaowalak;Abe, Hiroya;Fukumori, Yoshinobu
研究了模型蛋白（牛血清白蛋白，BSA）在商用羟基磷灰石（HAp）微球及其对照上的解吸行为。解吸行为与磷酸盐缓冲溶液中的磷酸盐浓度密切相关，随着磷酸盐浓度的增加，解吸BSA的量增加。在含有10 mM磷酸盐的生理缓冲液中，观察到牛血清白蛋白的初始爆发释放：0.5 h后快速解吸70%，24 h后快速解吸80%。相比之下，在蒸馏水中观察到牛血清白蛋白的极低释放。为了在生理条件下控制牛血清白蛋白的释放，采用固体-油-水（S/O/W）乳化溶剂蒸发法将装载牛血清白蛋白的HAp微球包裹在生物可降解聚合物聚乳酸-羟基乙酸（PLGA）中。包封显著降低了初始爆发，并显著延长了BSA释放时间。(C) 2007 Elsevier B.V.版权所有
Desorption behavior of a model protein (bovine serum albumin, BSA) on commercial hydroxyapatite (HAp) microspheres and its control were investigated for protein delivery system. The desorption behavior related strongly to the phosphate concentration in phosphate buffer solution: the amount of desorbed BSA increased when the phosphate concentration increased. In physiological buffer solution, which contains 10 mM phosphate, the initial burst release of BSA was observed: 70% of BSA was rapidly desorbed after 0.5 h, and 80% after 24 h. In contrast, the extremely low release profile of BSA was observed in distilled water. For the controlled release of BSA in physiological condition, the BSA-loaded HAp microspheres were encapsulated with a biodegradable polymer, poly(lactic acid-co-glycolic acid) (PLGA) by a solid-in oil-in water (S/O/W) emulsion solvent evaporation method. The initial burst was significantly reduced, and the BSA release was remarkably prolonged by the encapsulation. (C) 2007 Elsevier B.V. All rights reserved.