Genetic and Phenotypic Traits of Staphylococcus Epidermidis Strains Causing Postcataract Endophthalmitis Compared to Commensal Conjunctival Flora
Genetic and Phenotypic Traits of Staphylococcus Epidermidis Strains Causing Postcataract Endophthalmitis Compared to Commensal Conjunctival Flora
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DOI：
10.1016/j.ajo.2018.03.042
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发表时间：
2018-07-01
影响因子：
4.2
通讯作者：
Maurin, Max
中科院分区：
文献类型：
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作者：
Chiquet, Christophe;Musson, Cecile;Maurin, Max
目得：本研究的目的是比较的毒力和抗生素耐药性特征的表皮葡萄球菌菌株引起急性白内障后眼内炎的结膜分离的未感染的对照patients.DESIGN：病例对照study.METHODS：我们分离出的22眼内炎患者，并从43的72个对照组的表皮葡萄球菌菌株。使用基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱和tuf基因扩增和测序确认物种鉴定。使用AST-P631卡和Vitek II自动化系统评价抗生素亲和性。对表皮葡萄球菌菌株进行了7个毒力基因的检测结果：眼内炎患者的表皮葡萄球菌中aap、atlE和mecA基因携带率均高于对照组（分别为77% vs 42%，P = .007; 100% vs 79%，P = .02; 54% vs 11%，P < .001）。与对照组相比，他们也更频繁地携带mecA和icaA基因的组合（13% vs 2%，P = 0.01）。他们显着更耐甲氧西林，氟喹诺酮类药物，和aminoglycosides.CONCLUSIONS：一个更高的能力，粘附到人工透镜和生物膜的形成，以及更大的耐药性被发现在表皮葡萄球菌菌株引起白内障后眼内炎。这些毒力和抗生素耐药性标记物的有用性值得进一步评价，用于表皮葡萄球菌眼内炎的预防、治疗和预后评估。(C)2018爱思唯尔公司All rights reserved.
PURPOSE: The aim of this study was to compare the virulence and antibiotic resistance traits of Staphylococcus epidermidis strains causing acute postcataract endophthalmitis to those isolated from the conjunctiva of uninfected control patients.DESIGN: Case-control study.METHODS: We isolated an S epidermidis strain from each of the 22 endophthalmitis patients, and from 43 of the 72 controls. Species identification was confirmed using both Matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry and tuf gene amplification and sequencing. Antibiotic susceptibilities were evaluated using the AST-P631 card and the Vitek II automated system. The S epidermidis strains were tested for the presence of 7 virulence genes (icaA, icaB, icaC, icaD, atlE, aap, and capA), the insertion sequence 1S256, and the mecA gene.RESULTS: The S epidermidis strains from the endophthalmitis patients displayed higher prevalence rates for aap, atlE, and mecA gene carriage compared to those of the control group (77% vs 42%, P = .007; 100% vs 79%, P = .02; and 54% vs 11%, P < .001, respectively). They also harbored the combination of the mecA and icaA genes more frequently compared to the control group (13% vs 2%, P = .01). They were significantly more resistant than control strains to methicillin, fluoroquinolones, and the aminoglycosides.CONCLUSIONS: A higher capacity of adhesion to the intraocular lens and formation of biofilms as well as greater resistance to antibiotics were found in S epidermidis strains causing postcataract endophthalmitis. The usefulness of such virulence and antibiotic resistance markers warrants further evaluation for prevention, treatment, and prognostic evaluation of S epidermidis endophthalmitis. (C) 2018 Elsevier Inc. All rights reserved.