AtU2AF65b functions in abscisic acid mediated flowering via regulating the precursor messenger RNA splicing of ABI5 and FLC in Arabidopsis.
AtU2AF65b functions in abscisic acid mediated flowering via regulating the precursor messenger RNA splicing of ABI5 and FLC in Arabidopsis.
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DOI：
10.1111/nph.15756
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发表时间：
2019-03
期刊：
影响因子：
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通讯作者：
Feng Xiong;Jing-Jing Ren-Jing;Qin Yu;Yu-Yi Wang;Chongchong Lu;Lan-Jing Kong;M. Otegui;Xiu-Ling Wang
中科院分区：
文献类型：
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作者：
Feng Xiong;Jing-Jing Ren-Jing;Qin Yu;Yu-Yi Wang;Chongchong Lu;Lan-Jing Kong;M. Otegui;Xiu-Ling Wang
在哺乳动物和酵母中，剪接因子 U2AF65/Mud2p 在前体信使 RNA (pre-mRNA) 加工中发挥作用。拟南芥AtU2AF65b 编码假定的U2AF65，但其在植物中的具体功能尚不清楚。本文研究了 AtU2AF65b 作为拟南芥开花时间负调节因子的功能。我们研究了 AtU2AF65b 在脱落酸 (ABA) 调控开花中的表达和功能，以及 AtU2AF65b 敲除突变体中开花相关基因的转录本丰度和前体 mRNA 剪接。 atu2af65b 突变体在长日照和短日照条件下均表现出早花表型。由于内含子保留增加和转录激活减少，atu2af65b 茎尖中开花抑制基因 FLOWERING LOCUS C (FLC) 的转录积累减少。 FLC 转录减少至少部分是由于 ABSCISIC ACID-INSENSITIVE 5 (ABI5) 的异常剪接和转录本丰度减少造成的，ABI5 编码 ABA 调节的开花信号中 FLC 的激活剂。此外，AtU2AF65b 的表达受到 ABA 的促进。在 ABA 诱导的开花过程中，atu2af65b 突变体中向开花的转变以及 FLC 和 ABI5 的剪接受到损害。 ABA 响应性 AtU2AF65b 在茎尖的 FLC 和 ABI5 前 mRNA 剪接中发挥作用，从而 AtU2AF65b 参与拟南芥中 ABA 介导的开花转变。
In mammalians and yeast, the splicing factor U2AF65/Mud2p functions in precursor messenger RNA (pre-mRNA) processing. Arabidopsis AtU2AF65b encodes a putative U2AF65 but its specific functions in plants are unknown. This paper examines the function of AtU2AF65b as a negative regulator of flowering time in Arabidopsis. We investigated the expression and function of AtU2AF65b in abscisic acid (ABA)-regulated flowering as well as the transcript abundance and pre-mRNA splicing of flowering-related genes in the knock-out mutants of AtU2AF65b. The atu2af65b mutants show early-flowering phenotype under both long-day and short-day conditions. The transcript accumulation of the flowering repressor gene FLOWERING LOCUS C (FLC) is reduced in the shoot apex of atu2af65b, due to both increased intron retention and reduced transcription activation. Reduced transcription of FLC results, at least partially, from the abnormal splicing and reduced transcript abundance of ABSCISIC ACID-INSENSITIVE 5 (ABI5), which encodes an activator of FLC in ABA-regulated flowering signaling. Additionally, the expression of AtU2AF65b is promoted by ABA. Transition to flowering and splicing of FLC and ABI5 in the atu2af65b mutants are compromised during ABA-induced flowering. ABA-responsive AtU2AF65b functions in the pre-mRNA splicing of FLC and ABI5 in shoot apex, whereby AtU2AF65b is involved in ABA-mediated flowering transition in Arabidopsis.