Importance of CD8+Vβ8+ T cells in IFN-γ-mediated prevention of toxoplasmic encephalitis in genetically resistant BALB/c mice
Importance of CD8+Vβ8+ T cells in IFN-γ-mediated prevention of toxoplasmic encephalitis in genetically resistant BALB/c mice
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DOI：
10.1089/jir.2005.25.338
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发表时间：
2005-06-01
影响因子：
2.3
通讯作者：
Suzuki, Y
中科院分区：
文献类型：
--
作者：
Wang, XS;Claflin, J;Suzuki, Y
在我们试图鉴定识别保护性弓形虫抗原并产生γ-干扰素（IFN-γ）以预防弓形虫脑炎（TE）的主要T细胞群时，我们发现T细胞受体V β 8（+）细胞是最常见的浸润到T细胞脑中的产生IFN-γ的细胞群。感染弓形虫的BALB/c小鼠对该疾病具有遗传抗性。为了检测这种T细胞群产生IFN-γ对抵抗的作用，我们将从感染的BALB/c和IFN-γ（-/-）小鼠的脾脏中纯化的V β 8（+）免疫T细胞转移到感染的、磺胺嘧啶治疗的无胸腺裸鼠中。在磺胺嘧啶治疗停止后，未接受任何T细胞的对照裸鼠和接受来自IFN-γ（-/-）小鼠的V β 8（+）T细胞的动物都因感染再激活（TE）而死亡。相反，接受BALB/c小鼠细胞的动物存活了下来。因此，由V β 8（+）T细胞产生的IFN-γ在预防这些动物中的TE中起重要作用。当V β 8（+）免疫T细胞分为CD 4（+）和CD 8（+）亚群时，仅在CD 8+亚群中观察到有效的保护活性，而两个亚群的组合提供了比单独的CD 8（+）V β 8（+）群体更大的保护。这些结果表明，V β 8（+）T细胞的CD 8（+）亚群是IFN-γ介导的BALB/c小鼠对TE抗性的主要传入分支，尽管T细胞群的CD 4（+）亚群与CD 8（+）V β 8（+）群体具有相加或协同作用。
In our attempt to identify a major T cell population(s) that recognizes protective Toxoplasma gondii antigens and produces interferon-gamma (IFN-gamma) for prevention of toxoplasmic encephalitis (TE), we found T cell receptor V beta 8(+) cells to be the most frequent IFN-gamma-producing population infiltrated into the brain of T. gondii-infected BALB/c mice genetically resistant to the disease. To examine the role of IFN-gamma production by this T cell population for resistance, we transferred V beta 8(+) immune T cells purified from spleens of infected BALB/c and IFN-gamma(-/-) mice into infected, sulfadiazine-treated, athymic nude mice. After discontinuation of sulfadiazine treatment, control nude mice that had not received any T cells and animals that had received V beta 8(+) T cells from IFN-gamma(-/-) mice all died because of reactivation of infection (TE). In contrast, animals that had received the cells from BALB/c mice survived. Thus, IFN-gamma production by V beta 8(+) T cells plays an important role in prevention of TE in these animals. When V beta 8(+) immune T cells were divided into CD4(+) and CD8(+) subsets, a potent protective activity was observed only in the CD8+ subset, whereas a combination of both subsets provided greater protection than did the CD8(+)V beta 8(+) population alone. These results indicate that the CD8(+) subset of V beta 8(+) T cells is a major afferent limb of IFN-gamma-mediated resistance of BALB/c mice against TE, although the CD4(+) subset of the T cell population works additively or synergistically with the CD8(+)V beta 8(+) population.