Relaxin inhibits patellar tendon healing in rats: a histological and biochemical evaluation
Relaxin inhibits patellar tendon healing in rats: a histological and biochemical evaluation
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DOI：
10.1186/s12891-019-2729-3
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发表时间：
2019-07-27
影响因子：
2.3
通讯作者：
Geng, Dechun
中科院分区：
文献类型：
--
作者：
Xu, Tianpeng;Bai, Jiaxiang;Geng, Dechun
背景女性患者比男性患者更容易发生肌腱损伤，尤其是那些松弛素浓度较高的患者。先前的研究表明，松弛素可减弱细胞外基质 (ECM) 的形成。然而，松弛素对肌腱修复的作用机制仍不清楚。我们推测松弛素通过破坏胶原合成来抑制肌腱愈合。方法采用Sprague-Dawley大鼠建立髌腱窗缺损模型。从髌骨远端尖部取出髌腱中心，插入胫骨结节，宽度为1mm。然后，给大鼠注射生理盐水（0.2μg/kg/天）或松弛素（0.2μg/kg/天），持续两周和四周，然后进行生物力学分析和组织学和组织化学检查。结果力学结果表明，与未注射松弛素的大鼠相比，松弛素导致撕裂强度、刚度和杨氏模量显着下降。此外，研究表明松弛素可激活松弛素家族肽受体1（RXFP1），扰乱基质金属蛋白酶（MMP）和金属蛋白酶组织抑制剂（TIMP）之间的平衡，并减少损伤区域胶原蛋白的沉积。结论松弛素会损害大鼠的肌腱愈合。此外，松弛素可能导致女性比男性更常见的肌腱损伤。
BackgroundFemale patients are more likely to have tendon injuries than males, especially those who has a higher concentration of relaxin. Previous studies have demonstrated that relaxin attenuates extracellular matrix (ECM) formation. However, the mechanism of relaxin on tendon repair remains unclear. We hypothesize that relaxin inhibits tendon healing by disrupting collagen synthesis.MethodsA patellar tendon window defect model was established using Sprague-Dawley rats. The center of the patellar tendon was removed from the patella distal apex and inserted to the tibia tuberosity in width of 1mm. Then, the rats were injected with saline (0.2 mu g/kg/day) or relaxin (0.2 mu g/kg/day) for two and four weeks, which was followed by biomechanical analysis and histological and histochemical examination.ResultsMechanical results indicated that relaxin induces a significant decrease in tear resistance, stiffness, and Young's modulus compared to those rats without relaxin treatment. In addition, it was shown that relaxin activates relaxin family peptide receptor 1(RXFP1), disturbs the balance between matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteases (TIMPs), and reduces the deposition of collagen in injury areas.ConclusionsRelaxin impairs tendon healing in rats. Also, relaxin might lead to tendon injury more commonly for females than males.