Genomic homology of the domestic ferret with cats and humans
Genomic homology of the domestic ferret with cats and humans
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DOI：
10.1007/s003350010172
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发表时间：
2000-10-01
期刊：
影响因子：
2.5
通讯作者：
Stanyon, R
中科院分区：
文献类型：
--
作者：
Cavagna, P;Menotti, A;Stanyon, R
我们使用家猫和人类的染色体油漆直接建立这些物种与家貂基因组之间的染色体同源性。染色体彩绘数据表明，雪貂的核型比猫的核型更接近于祖先食肉动物的核型。猫染色体彩绘揭示了雪貂基因组中22个同源的常染色体区域：16条雪貂染色体被一个猫彩绘杂交，3条雪貂染色体被两个猫彩绘杂交。原位杂交结合显带分析表明，雪貂染色体（Chr）1为cat A2 p/C2，Chr 2为cat F2/C1 q，Chr 3为cat A2 q/D2。5条雪貂染色体与猫染色体的单臂同源：雪貂4 = A1 q，5 = Big，6 = Clp，10 = Alp，12 = B1 p。人类染色体涂料揭示了雪貂基因组中的32 + XY同源区域：9条雪貂染色体分别与一种人类涂料杂交，7条与两种涂料杂交，3条与三种涂料杂交。10条雪貂染色体与多个人类染色体杂交产生以下关联：雪貂1 =人类19/3/21，2 = 8 q/2 q，3 = 10/7，5 = 8/4，8 = 15/14，9 = 10/12/22，11 = 20/2，12 = 8/4，14 = 12/22/18，18 = 19/16。我们提出了一个指数的基因组多样性，Z，保守的整个染色体和染色体片段的相对数量的基础上，作为一个初步的统计物种之间的快速比较。人-雪貂间的多样性指数（Z = 0.812）略小于人-猫间的多样性指数（Z = 0.843）。这里提供的同源性数据使我们能够将猫和人类的基因定位数据转移到雪貂身上。
We used chromosome paints from both the domestic cat and humans to directly establish chromosomal homology between the genome of these species and the domestic ferret. The chromosome painting data indicate that the ferret has a highly conserved karyotype closer to the ancestral carnivore karyotype than that of the cat. The cat chromosome paints revealed 22 homologous autosomal regions in the ferret genome: 16 ferret chromosomes were hybridized by a single cat paint, while 3 ferret chromosomes were hybridized by two cat paints. In situ hybridization combined with banding showed that ferret Chromosome (Chr) 1 = cat A2p/C2, Chr 2 = F2/C1q, and Chr 3 = A2q/D2. Five ferret chromosomes are homologous to single arms of cat chromosomes: ferret 4 = A1q, 5 = Big, 6 = Clp, 10 = Alp, and 12 = B1p. The human chromosome paints revealed 32 + XY homologous regions in the ferret genome: 9 ferret chromosomes were each hybridized by a single human paint, 7 by two paints, 3 by three paints. The 10 ferret chromosomes hybridized by multiple human paints produced the following associations: ferret 1 = human 19/3/21, 2 = 8q/2q, 3 = 10/7,5 = 8/4, 8 = 15/14, 9 = 10/12/22, 11 = 20/2, 12 = 8/4, 14 = 12/22/18, 18 = 19/16. We present an index of genomic diversity, Z, based on the relative number of conserved whole chromosome and chromosome segments as a preliminary statistic for rapid comparison between species. The index of diversity between human-ferret (Z = 0.812) is slightly less than human-cat (Z = 0.843). The homology data presented here allow us to transfer gene mapping data from both cats and humans to the ferret.