p27kip1 deficiency accelerates dentin and alveolar bone formation
p27kip1 deficiency accelerates dentin and alveolar bone formation
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p27(kip1) 缺乏会加速牙本质和牙槽骨的形成
DOI：
10.1111/1440-1681.12276
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发表时间：
2014-10-01
影响因子：
2.9
通讯作者：
Miao, Dengshun
中科院分区：
文献类型：
--
作者：
Yin, Ying;Wang, Qun;Miao, Dengshun
为了评估p27（kip 1）在调节牙齿形成和牙槽骨发育中的作用，我们比较了2周龄时纯合子p27（kip 1）缺陷（p27（-/-））小鼠与其野生型同窝小鼠的牙齿和下颌骨表型。与野生型（WT）小鼠相比，p27（-/-）小鼠的牙体密度、牙体体积和牙本质涎蛋白免疫阳性面积显著增加，而前牙本质面积：牙本质总面积和双糖蛋白免疫阳性面积：牙本质面积比值显著降低。下颌骨的骨密度、皮质厚度、牙槽骨体积、I型胶原和osterix免疫阳性面积、成骨细胞数量和活性以及Runt相关转录因子2（Runx 2）、碱性磷酸酶（ALP）、骨钙素和骨形态发生蛋白（bmp 2）的mRNA表达均显著增加，p27（-/-）小鼠牙槽骨中抗酒石酸酸性磷酸酶阳性破骨细胞的数量和表面与WT同窝小鼠相比也是如此。此外，增殖细胞核抗原阳性细胞的百分比在Hertwig的上皮根鞘和蛋白表达的细胞周期蛋白E和细胞周期蛋白依赖性激酶2在p27（-/-）小鼠相对于其WT同窝仔显着增加。本研究结果提示p27在牙本质形成和牙槽骨发育中起负性调节作用。
To assess the role of p27(kip1) in regulating dental formation and alveolar bone development, we compared the teeth and mandible phenotypes of homozygous p27(kip1)-deficient (p27(-/-)) mice with their wild-type littermates at 2weeks of age. At 2weeks of age, dental mineral density, dental volume and dentin sialoprotein-immunopositive areas were increased significantly, whereas the predentin area:total dentin area and biglycan-immunopositive area:dentin area ratios were decreased significantly in p27(-/-) mice compared with their wild-type (WT) littermates. Mandible mineral density, cortical thickness, alveolar bone volume, type I collagen and osterix-immunopositive areas, osteoblast number and activity and mRNA expression of Runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP), osteocalcin and bone morphogenetic protein (bmp2) were all significantly increased in the mandibles, as was the number and surface of tartrate-resistant acid phosphatase-positive osteoclasts in the alveolar bone of p27(-/-) mice compared with their WT littermates. Furthermore, the percentage of proliferating cell nuclear antigen-positive cells in Hertwig's epithelial root sheath and protein expression of cyclin E and cyclin-dependent kinase 2 were increased significantly in p27(-/-) mice relative to their WT littermates. The results from this study indicate that p27 plays a negative regulatory role in dentin formation and alveolar bone development.