Biochemical analysis of new type mutants of japonica rice that accumulate water-soluble α-glucans in the endosperm but retain full starch debranching enzyme activities
Biochemical analysis of new type mutants of japonica rice that accumulate water-soluble α-glucans in the endosperm but retain full starch debranching enzyme activities
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胚乳中积累水溶性α-葡聚糖但保留完整淀粉脱支酶活性的粳稻新型突变体的生化分析
DOI：
10.1002/star.201600159
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发表时间：
2017
期刊：
影响因子：
2.3
通讯作者：
Yasunori Nakamura
中科院分区：
文献类型：
--
作者：
Tsuyoshi Nakagami;Hiroki Yoshihara;Tetsuhiro Nakamura;Yoshinori Utsumi;T. Sawada;N. Fujita;H. Satoh;Yasunori Nakamura
支链淀粉具有高度调节的支化结构，称为簇结构，其导致葡聚糖疏水并形成淀粉颗粒的半结晶结构。已知异淀粉酶1（ISA 1）基因的损伤导致称为植物糖原的水溶性葡聚糖（WSG）而不是支链淀粉在各种植物物种中的积累。这种类型的谷物突变体被称为糖-1，并在胚乳中积累大量的植物糖原。在本研究中，从粳稻中分离到了另一个合成WSG的突变体。该突变体在胚乳中心积累了大量的WSG。伊萨和普鲁兰酶的活性以及ISA 1同聚体和ISA 1-ISA 2异聚体的组成没有明显变化。此外，淀粉分支酶和淀粉合成酶同工酶的活性没有改变。积累的WSG具有不同于植物糖原的特殊精细结构。因此，我们将这种新的突变体命名为糖-2突变体。我们的研究结果表明，Sugary-2基因产物在水稻胚乳支链淀粉合成中起重要作用，并确定了一个新的控制支链淀粉正常结构的因子，特别是在胚乳发育的早期。
Amylopectin has a highly regulated branched structure called cluster structure, which causes the glucan to be hydrophobic and to form a semicrystalline architecture of starch granules. It is known that lesions of the isoamylase1 (ISA1) gene result in accumulation of a water-soluble glucan (WSG) called phytoglycogen instead of amylopectin in various plant species. This type of cereal mutant is referred to as sugary-1 and accumulates a large amount of phytoglycogen in the endosperm. In this study, another WSG-synthesizing mutant has been isolated from japonica rice. This mutant accumulated a significant amount of WSG in the center of endosperm. No significant changes were found in activities of ISA and pullulanase and in the compositions of ISA1 homomer and ISA1-ISA2 heteromer. In addition, activities of starch branching enzyme and starch synthase isoforms were not altered. The accumulated WSG had a specific fine structure, that differed from that of phytoglycogen. Thus, we designated this new mutant as the sugary-2 mutant. Our study results strongly suggest that the Sugary-2 gene product plays an important role in amylopectin synthesis of rice endosperm and identifies a new factor that controls the normal amylopectin structure, especially at the early developmental stage of the endosperm.
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影响因子：
7.4
作者：
Fujita, N;Yoshida, M;Nakamura, Y
通讯作者：
Nakamura, Y
影响因子：
13.8
作者：
Tanaka, N;Fujita, N;Nakamura, Y
通讯作者：
Nakamura, Y
影响因子：
3.8
作者：
Wong, KS;Kubo, A;Nakamura, Y
通讯作者：
Nakamura, Y
影响因子：
7.4
作者：
Kubo, A;Rahman, S;Nakamura, Y
通讯作者：
Nakamura, Y
水稻中 MNU 处理的饱和诱变。
DOI：
--
发表时间：
2009
期刊：
影响因子：
--
作者：
Yoshimori T;Amano A.;佐藤光
通讯作者：
佐藤光