Effect of nitric oxide on renin secretion. II. Studies in the perfused juxtaglomerular apparatus.
Effect of nitric oxide on renin secretion. II. Studies in the perfused juxtaglomerular apparatus.
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一氧化氮对肾素分泌的影响。
DOI：
10.1152/ajprenal.1995.268.5.f953
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Schnermann,JB
中科院分区：
文献类型：
--
作者：
He,XR;Greenberg,SG;Briggs,JP;Schnermann,JB
为了研究NO在致密斑控制肾素分泌中的可能作用，我们研究了不同的NO可用性对离体灌注兔肾小球旁器（JGA）中肾素释放的影响。管腔 Na/Cl 浓度比 (mM/mM) 从 26/7 逐渐增加到 46/27、66/47 至 86/67，导致肾素分泌逐渐减少，从（纳米 Goldblatt hog 单位的对数与时间的对数，即 log nGU/min）1.09 +/- 0.34 到 0.46 +/- 0.24 log nGU/min，其中最大的变化发生在第一个浓缩步骤。管腔液中存在 0.7 mM N omega-硝基-L-精氨酸 (NNA)（一种 NO 合酶抑制剂），在最低 Na/Cl 浓度比为 0.65 +/- 0.32 log nGU/min 时显着减少肾素分泌（与对照相比，P < 0.01）。与对照相比，NNA 在较高 Na/Cl 浓度比下的肾素分泌没有显着影响。与这些结果相反，在浴液中添加 NO 供体硝普钠 (1 mM) 导致肾素分泌量从 1.0 +/- 0.39 减少至 0.47 +/- 0.46 log nGU/min (P < 0.05)，通过在浴液中添加 0.01 mM 亚甲基蓝可逆转这一效应。同样，向浴中添加 L-精氨酸 (0.7 mM) 使肾素分泌从 0.99 +/- 0.37 减少至 0.81 +/- 0.38 log nGU/min (P < 0.01)，而向腔液中添加 L-精氨酸则使肾素分泌从 0.85 +/- 0.43 增加至 1.94 +/- 0.46 log nGU/min (P < 0.01)。 0.05）。添加 NNA 可逆转 Luminal L-精氨酸的刺激作用。（摘要截断为 250 字）
To examine the possible role of NO in macula densa control of renin secretion, we examined the effects of varying NO availability on renin release in the isolated perfused rabbit juxtaglomerular apparatus (JGA). Gradual increments of luminal Na/Cl concentration ratio (mM/mM) from 26/7 over 46/27, 66/47, to 86/67 caused a progressive decrease in renin secretion from (as log of nano-Goldblatt hog units vs. time, i.e., log nGU/min) 1.09 +/- 0.34 to 0.46 +/- 0.24 log nGU/min, with the greatest change occurring at the first concentration step. The presence of 0.7 mM N omega-nitro-L-arginine (NNA), an NO synthase inhibitor, in the luminal fluid significantly reduced renin secretion at the lowest Na/Cl concentration ratio to 0.65 +/- 0.32 log nGU/min (P < 0.01 compared with control). Renin secretion at the higher Na/Cl concentration ratios was not significantly affected by NNA compared with control. In contrast to these results, the addition of the NO donor nitroprusside (1 mM) to the bath caused a reduction in renin secretion from 1.0 +/- 0.39 to 0.47 +/- 0.46 log nGU/min (P < 0.05), an effect that was reversed by bath addition of 0.01 mM methylene blue. Similarly, addition of L-arginine (0.7 mM) to the bath reduced renin secretion from 0.99 +/- 0.37 to 0.81 +/- 0.38 log nGU/min (P < 0.01), whereas addition of L-arginine to the luminal fluid increased renin secretion from 0.85 +/- 0.43 to 1.94 +/- 0.46 log nGU/min (P < 0.05). The stimulatory effect of luminal L-arginine was reversed by the luminal addition of NNA.(ABSTRACT TRUNCATED AT 250 WORDS)