Determining the nucleotide sequence and capsid-coding region of Himetobi P virus: a member of a novel group of RNA viruses that infect insects
Determining the nucleotide sequence and capsid-coding region of Himetobi P virus: a member of a novel group of RNA viruses that infect insects
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DOI：
10.1007/s007050050726
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发表时间：
1999-01-01
影响因子：
2.7
通讯作者：
Toriyama, S
中科院分区：
文献类型：
--
作者：
Nakashima, N;Sasaki, J;Toriyama, S
本研究测定了从灰飞虱体内分离到的一种昆虫小核糖核酸样病毒Himeplani P病毒（HiPV）的全基因组序列。HiPV的基因组由9,275个核苷酸组成，不包括poly（A）rail，并且包含两个大的开放阅读框架（ORF），其由176个核苷酸的非编码区隔开。推导的第一个ORF的氨基酸序列包含小核糖核酸病毒解旋酶，蛋白酶和RNA依赖的RNA聚合酶的核心基序。通过确定衣壳蛋白的N-末端氨基酸序列，将衣壳蛋白编码区定位到第二个ORF上。在感染组织中未检测到衣壳蛋白基因的亚基因组RNA。HiPV衣壳蛋白前体基因在预期位置缺少一个AUG起始密码子，并且该基因的上游序列被预测为形成多个茎环结构，这表明该前体基因是通过内部核糖体进入位点（IRES）介导的翻译产生的，就像Plutia stall intestinal virus（PSIV）一样。HiPV基因组的这些特征类似于由果蝇C病毒（DCV）、禾谷缢管蚜病毒（RhPV）和PSIV组成的一组新的RNA病毒。
We determined the complete genome sequence of Himetobi P virus (HiPV), an insect picorna-like virus, which was isolated from the small brown planthopper, Laodelphax striatellus. The genome of HiPV consists of 9,275 nucleotides excluding the poly (A) rail, and contains two large open reading frames (ORFs), which were separated by a 176-nucleotide noncoding region. The deduced amino acid sequence of the first ORF contains core motifs of picornaviral helicase, protease, and RNA-dependent RNA polymerase. The capsid protein-coding region was mapped onto the second ORF by determining the N-terminal amino acid sequences of the capsid proteins. Subgenomic RNA for the capsid protein gene was not detected in the infected tissue. The capsid protein precursor gene of HiPV lacks an AUG initiation codon at the expected position and the upstream sequence of the gene is predicted to form several stem-loop structures, suggesting that the precursor is produced by internal ribosome entry site (IRES) mediated-translation, as occurs in Plutia stall intestine virus (PSIV). These characteristics of the HiPV genome are similar to those of a new group of RNA viruses consisting of Drosophila C virus (DCV), Rhopalosiphum padi virus (RhPV), and PSIV.