Catechol oestradiol control of FSH-stimulated granulosa cell steroidogenesis.
Catechol oestradiol control of FSH-stimulated granulosa cell steroidogenesis.
复制标题
儿茶酚雌二醇控制 FSH 刺激的颗粒细胞类固醇生成。
作者：
K. E. Hudson;S. Hillier
儿茶酚类雌激素在卵巢中形成，但不知道它们是否有局部功能。利用未成熟大鼠卵巢制备的原代颗粒细胞培养物，我们发现培养基中2-羟基雌二醇（48 h）的存在显著增强了对人卵泡刺激素（100 ng/ml）的类固醇生成反应（黄体酮生成）。2-羟基雌二醇的作用是剂量依赖性的，在3.0微米时达到最大（约为FSH单独作用的40倍）。1.0 μ m 2-羟基雌二醇的刺激作用是1.0 μ m雌二醇的10倍以上，而仅为1.0 μ m睾酮的一半；其他儿茶酚类雌激素（2-羟基雌酮、4-羟基雌二醇、2-甲氧基雌二醇和2-甲氧基雌酮）没有刺激作用。2-羟基雌二醇和睾酮的刺激作用部分是加在一起的，它们通过一种特定的抗雄激素（SCH16423）以同样的方式被拮抗。这些观察结果表明，卵泡内儿茶酚雌二醇在调节fsh刺激的颗粒细胞类固醇生成中的作用；其作用机制可能与睾酮相似。
Catechol oestrogens are formed in the ovary but it is not known if they have a local function. Working with primary granulosa cell cultures prepared from immature rat ovaries, we found that the presence of 2-hydroxyoestradiol in the culture medium (48 h incubation) dramatically enhanced the steroidogenic response (progesterone production) to human FSH (100 ng/ml). The effect of 2-hydroxyoestradiol was dose-dependent and maximal (approximately 40 times the response to FSH alone) at 3.0 microM. The stimulatory action of 1.0 microM 2-hydroxyoestradiol was greater than 10 times more than that of 1.0 microM oestradiol but only half that of 1.0 microM testosterone; other catechol oestrogens (2-hydroxyoestrone, 4-hydroxyoestradiol, 2-methoxyoestradiol and 2-methoxyoestrone) were not stimulatory. The stimulatory actions of 2-hydroxyoestradiol and testosterone were partially additive and each was antagonized in the same way by the presence of a specific antiandrogen (SCH16423). These observations suggest a role for intrafollicular catechol oestradiol in modulating FSH-stimulated granulosa cell steroidogenesis; its mechanism of action may be similar to that of testosterone.