Structural changes accompanying GTP hydrolysis in microtubules: information from a slowly hydrolyzable analogue guanylyl-(alpha,beta)-methylene-diphosphonate.
Structural changes accompanying GTP hydrolysis in microtubules: information from a slowly hydrolyzable analogue guanylyl-(alpha,beta)-methylene-diphosphonate.
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微管中 GTP 水解伴随的结构变化：来自缓慢水解类似物鸟苷基-(α,β)-亚甲基二膦酸酯的信息。
DOI：
10.1083/jcb.128.1.117
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发表时间：
1995-01
期刊：
影响因子：
--
通讯作者：
Wade RH
中科院分区：
文献类型：
--
作者：
Hyman AA;Chrétien D;Arnal I;Wade RH
我们已经使用低温电子显微镜试图了解GTP水解在破坏微管晶格稳定中的作用的结构基础。我们测量了引入微管的结构差异，通过用胍基- (α, β)-亚甲基二膦酸盐（GMPCPP）取代GTP。在稳定的GMPCPP微管晶格中，云纹图案发生变化，微管蛋白亚基的尺寸增加了1.5 a。这一信息为水解在诱导微管末端从生长阶段到收缩阶段的结构变化中所起的作用提供了线索。
We have used cryoelectron microscopy to try to understand the structural basis for the role of GTP hydrolysis in destabilizing the microtubule lattice. We have measured a structural difference introduced into microtubules by replacing GTP with guanylyl- (alpha,beta)-methylene-diphosphonate (GMPCPP). In a stable GMPCPP microtubule lattice, the moire patterns change and the tubulin subunits increase in size by 1.5 A. This information provides a clue to the role of hydrolysis in inducing the structural change at the end of a microtubule during the transition from a growing to a shrinking phase.