Tunable optical setup with high flexibility for spectrally resolved coherent anti-Stokes Raman scattering microscopy
Tunable optical setup with high flexibility for spectrally resolved coherent anti-Stokes Raman scattering microscopy
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DOI：
10.1002/lapl.201110026
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发表时间：
2011-07-01
影响因子：
1.7
通讯作者：
Popp, J.
中科院分区：
文献类型：
--
作者：
Bergner, G.;Akimov, D.;Popp, J.
A simplified setup for coherent anti-Stokes Raman scattering (CARS) microscopy is introduced, which allows for recording CARS images with 30 cm(-1) excitation bandwidth for probing Raman bands between 500 and 900 cm(-1) with minimal requirements for alignment. The experimental arrangement is based on electronic switching between CARS images recorded at different Raman resonances by combining a photonic crystal fiber (PCF) as broadband light source and an acousto-optical programmable dispersive filter (AOPDF) as tunable wavelength filter. Such spatial light modulator enables selection of a narrow-band spectrum to yield high vibrational contrast and hence chemical contrast in the resultant CARS images. Furthermore, an experimental approach to reconstruct spectral information from CARS image contrast is introduced.
