PCR primers for the amplification of four insect mitochondrial gene fragments
PCR primers for the amplification of four insect mitochondrial gene fragments
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DOI：
10.1111/j.1365-2583.1995.tb00028.x
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发表时间：
1995-11-01
影响因子：
2.6
通讯作者：
Smith, PT
中科院分区：
文献类型：
--
作者：
Kambhampati, S;Smith, PT
昆虫线粒体基因组（mtDNA）分析是研究群体遗传学和系统发育学的有力工具。在过去的几年里，用于各种昆虫 mtDNA 基因 PCR 扩增的引物序列已经发表。本研究的目的是（1）提出六种昆虫线粒体基因的新引物序列，以及（2）测试我们实验室设计的引物和一些先前发表的针对多种昆虫的引物，以确定是否可以获得目标片段的扩增。用于扩增两个核糖体RNA基因（16S和12S rRNA）片段的引物是昆虫及相关类群通用的； NADH5和NADH4脱氢酶基因片段和细胞色素c氧化酶I基因片段的引物适用范围广泛。
Insect mitochondrial genome (mtDNA) analysis is a powerful tool for the study of population genetics and phylogenetics. In the past few years primer sequences for the PCR amplification of various insect mtDNA genes have been published. The objectives of this study were (1) present new primer sequences for six insect mitochondrial genes and (2) test primers designed in our laboratory and some previously published primers on a wide range of insects to determine if amplification of the target fragment could be obtained. The primers for the amplification of the two ribosomal RNA gene (16S and 12S rRNA) fragments are universal for insects and related groups; the primers for NADH5 and NADH4 dehydrogenase gene fragments and cytochrome c oxidase I gene fragment are applicable broadly.