SELECTIVITY OF YOLK PROTEIN UPTAKE - COMPARISON OF VITELLOGENINS OF 2 INSECTS
SELECTIVITY OF YOLK PROTEIN UPTAKE - COMPARISON OF VITELLOGENINS OF 2 INSECTS
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DOI：
10.1016/0022-1910(76)90248-1
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发表时间：
1976-01-01
影响因子：
2.2
通讯作者：
PAN, ML
中科院分区：
文献类型：
--
作者：
KUNKEL, JG;PAN, ML
盲鳗和德国小蠊的卵黄原蛋白具有相似的Stokes半径（69和75.ANG）、沉降系数（15.9和16.8S）和等电点（pH分别为5.7和5.0），氨基酸组成与其他动物的卵黄原蛋白相似。2种卵黄原蛋白无免疫交叉反应。在体内，小蠊卵母细胞吸收自身卵黄原蛋白的速度很快，而透明眼卵母细胞吸收自身卵黄原蛋白的速度较低，与非卵黄原蛋白相当。玻璃体卵母细胞在体外快速吸收自身的卵黄原蛋白，而小蠊卵母细胞吸收卵黄原蛋白的速度可以忽略不计。卵黄蛋白原的分子量、形状和电荷与非卵黄蛋白原相似，不形成选择性摄取的基础。
The vitellogenins of Hyalophora cecropia and Blattella germanica have similar Stokes'' radii (69 and 75.ANG.), sedimentation coefficients (15.9 and 16.8S) and isoelectric points (pH 5.7 and 5.0), and share similar amino acid compositions with vitellogenins of other animals. The 2 vitellogenins show no immunological crossreaction. Blattella oocytes take up their own vitellogenin in vivo at a rapid rate and that of Hyalophora at a low rate comparable to that of a non-vitellogenic protein. Hyalophora oocytes take up their own vitellogenin rapidly in vitro and Blattella vitellogenin only at a negligible rate. Molecular weights, shapes and charges of the vitellogenins are similar to non-vitellogenins and form no basis for selective uptake.