Characterization of circulating endothelial cells in acute myocardial infarction.
Characterization of circulating endothelial cells in acute myocardial infarction.
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DOI：
10.1126/scitranslmed.3003451
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发表时间：
2012-03-21
影响因子：
17.1
通讯作者：
Topol EJ
中科院分区：
文献类型：
--
作者：
Damani S;Bacconi A;Libiger O;Chourasia AH;Serry R;Gollapudi R;Goldberg R;Rapeport K;Haaser S;Topol S;Knowlton S;Bethel K;Kuhn P;Wood M;Carragher B;Schork NJ;Jiang J;Rao C;Connelly M;Fowler VM;Topol EJ
急性心肌梗死(MI)涉及现有动脉粥样斑块的破裂，尽管最近在冠状动脉疾病的诊断和治疗方面取得了进展，但仍具有高度的不可预测性。因此，迫切需要一种能够预测即将发生的心肌梗死的生物标志物。在这里，我们使用第一个自动化和临床可行的循环内皮细胞(CEC)三通道荧光显微镜分析方法，对50例连续的ST段抬高心肌梗死(STEMI)患者和44例连续的健康对照进行了循环内皮细胞(CEC)的特征分析。心肌梗死组CEC计数明显高于对照组，中位数分别为19和4个/ml(p=1.1×10−10)。受试者-操作特征(ROC)曲线分析显示ROC曲线下的面积为0.95，这表明MI患者和对照组几乎一分为二。未观察到CEC与心肌坏死的典型标志物之间的相关性(ρ=0.02，CK-MB；ρ=−=0.03，肌钙蛋白)。显微图像的形态分析显示，与健康对照组、年龄匹配的CEC以及来自既有外周血管疾病患者的CEC相比，MI CEC的细胞面积增加了2.5倍(P<；0.0001)，细胞核面积增加了2倍(P<；0.0001)。含2~10个核的CEC图像的分布表明，MI患者是唯一一个包含3个以上核/像的组，表明多细胞和多核簇是急性MI的特异性。这些数据表明CEC可作为预测动脉粥样硬化斑块破裂事件的有前景的生物标志物。
Acute myocardial infarction (MI), which involves the rupture of existing atheromatous plaque, remains highly unpredictable despite recent advances in the diagnosis and treatment of coronary artery disease. Accordingly, a biomarker that can predict an impending MI is desperately needed. Here, we characterize circulating endothelial cells (CECs) using the first automated and clinically feasible CEC 3-channel fluorescence microscopy assay in 50 consecutive patients with ST-elevation myocardial infarction (STEMI) and 44 consecutive healthy controls. CEC counts were significantly elevated in MI cases versus controls with median numbers of 19 and 4 cells/ml respectively (p = 1.1 × 10−10). A receiver-operating characteristic (ROC) curve analysis demonstrated an area under the ROC curve of 0.95, suggesting near dichotomization of MI cases versus controls. We observed no correlation between CECs and typical markers of myocardial necrosis (ρ=0.02, CK-MB; ρ=−0.03, troponin). Morphologic analysis of the microscopy images of CECs revealed a 2.5-fold increase (P<0.0001) in cellular area and 2-fold increase (P<0.0001) in nuclear area of MI CECs versus healthy control, age-matched CECs, as well as CECs obtained from patients with preexisting peripheral vascular disease. The distribution of CEC images containing from 2 up to 10 nuclei demonstrates that MI patients are the only group to contain more than 3 nuclei/image, indicating that multi-cellular and multi-nuclear clusters are specific for acute MI. These data indicate that CECs may serve as promising biomarkers for the prediction of atherosclerotic plaque rupture events.
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影响因子：
37.8
作者：
Quilici, J;Banzet, N;Dignat-George, F
通讯作者：
Dignat-George, F
影响因子：
4.8
作者：
Tavazzi, L
通讯作者：
Tavazzi, L
影响因子：
158.5
作者：
Braunwald, E
通讯作者：
Braunwald, E
影响因子：
24
作者：
Sluimer, Judith C.;Kolodgie, Frank D.;Bijnens, Ann P. J. J.;Maxfield, Kimberly;Pacheco, Erica;Kutys, Bob;Duimel, Hans;Frederik, Peter M.;van Hinsbergh, Victor W. M.;Virmani, Renu;Daemen, Mat J. A. P.
通讯作者：
Daemen, Mat J. A. P.
影响因子：
39.3
作者：
Boos, Christopher J.;Soor, Simren K.;Lip, Gregory Y. H.
通讯作者：
Lip, Gregory Y. H.