Improvement of postnatal neovascularization by human adipose tissue-derived stem cells
Improvement of postnatal neovascularization by human adipose tissue-derived stem cells
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DOI：
10.1161/01.cir.0000135466.16823.d0
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发表时间：
2004-07-20
期刊：
影响因子：
37.8
通讯作者：
Bouloumié, A
中科院分区：
文献类型：
--
作者：
Miranville, A;Heeschen, C;Bouloumié, A
背景-几项研究表明，干细胞存在于脂肪组织（AT）的基质-血管部分（SVF）中。方法和结果-为了表征来自皮下和内脏贮库的构成人AT的SVF的细胞群，通过使用针对内皮和干细胞标记物CD 31、CD 34、CD 133、CD 134和CD 135的荧光抗体进行荧光激活细胞分选仪分析。ABCG2。新鲜收获的SVF含有大量的CD 34（+）细胞以及表达CD 133和ABCG 2的细胞。对CD 34（+）细胞的进一步分析显示2个CD 34（+）细胞群具有内皮细胞标志物CD 31的差异表达。通过使用磁性微珠选择CD 34（+）/CD 31（-）细胞，随后进行细胞培养，证明该细胞群在适当条件下可分化为内皮细胞。此外，在小鼠缺血后肢，静脉注射的CD 34（+）/CD 31（-）细胞与血流量和毛细血管密度的增加，并在腿部vasculations.Conclusions-Our数据表明，存在的细胞群内的SVF的人AT表征为CD 34（+）/CD 31（-）表现出内皮祖细胞的特性。因此，人AT可能代表用于细胞疗法以改善成人血管发生的干/祖细胞的来源。
Background-Several studies have suggested that stem cells are present in the stroma-vascular fraction (SVF) of adipose tissue ( AT).Methods and Results-To characterize the cell populations that compose the SVF of human AT originating from subcutaneous and visceral depots, fluorescence-activated cell sorter analysis was performed by use of fluorescent antibodies directed against the endothelial and stem cell markers CD31, CD34, CD133, and ABCG2. The freshly harvested SVF contained large numbers of CD34(+) cells as well as cells expressing CD133 and ABCG2. Further analysis of the CD34(+) cells revealed 2 CD34(+) cell populations with differential expression of the endothelial cell marker CD31. Selection of the CD34(+)/CD31(-) cells by use of magnetic microbeads, followed by cell culture, demonstrated that this cell population could differentiate under appropriate conditions into endothelial cells. Moreover, in mouse ischemic hindlimb, intravenous injection of CD34(+)/CD31(-) cells was associated with an increase in the blood flow and the capillary density and an incorporation of the cells in the leg vasculature.Conclusions-Our data indicate the presence of a cell population within the SVF of human AT characterized as CD34(+)/CD31(-) exhibiting characteristics of endothelial progenitor cells. Therefore, human AT might represent a source of stem/progenitor cells useful for cell therapy to improve vasculogenesis in adults.