Ultrastructural observations of fibroblast-like cells forming gap junctions in the W/Wv mouse small intestine
Ultrastructural observations of fibroblast-like cells forming gap junctions in the W/Wv mouse small intestine
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DOI：
10.1016/s0165-1838(00)00089-8
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发表时间：
2000-05-12
期刊：
影响因子：
--
通讯作者：
Komuro, T
中科院分区：
文献类型：
--
作者：
Horiguchi, K;Komuro, T
将野生型（+/+）小鼠小肠的超微结构与仅具有少量与奥尔巴赫氏神经丛相关的Cajal间质细胞（ICC）的c-kit突变型（W/W-nu）小鼠进行比较，以阐明专门的膜接触是否是广泛分布于消化道的图尼卡肌层中的所谓成纤维细胞样细胞的一般特征。在W/W-nu小鼠和+/+小鼠中发现奥尔巴赫区的成纤维细胞样细胞的数量大致相等，而在本研究中，W/W-nu小鼠中不能证明与奥尔巴赫神经丛相关的ICC（ICC-AP）。成纤维细胞样细胞的特点是细胞质中到高电子密度，发达的粗面内质网和细胞核与厚的异染色质聚集的周边。细胞膜无基膜和沿着排列的小窝。观察到单个成纤维细胞样细胞与环形和纵向层的肌细胞形成可能的小缝隙连接。在+/+和W/W-nu小鼠的深肌丛区域也观察到具有相同特征的成纤维细胞样细胞。本研究结果以及我们以前对大鼠和豚鼠的研究结果表明，胃肠道肌肉组织中成纤维细胞样细胞上普遍存在缝隙连接或缝隙连接样结构，它们通过传递电信号或分子信号影响肌肉紧张状态，参与胃肠道运动的调节系统。(C)2000 Elsevier Science B. V.保留所有权利。
The ultrastructure of the wild-type (+/+) mice small intestine was compared with c-kit mutant (W/W-nu) mice which only have few interstitial cells of Cajal (ICC) associated with Auerbach's plexus, in order to elucidate whether the specialized membrane contacts are general features of so-called fibroblast-like cells that are widely distributed in the tunica muscularis of the alimentary tract. Fibroblast-like cells in the Auerbach region were found in approximately equal number in W/W-nu mice as in +/+ mice, while ICC associated with Auerbach's plexus (ICC-AP) could not be demonstrated in W/W-nu mice in the present investigation. Fibroblast-like cells were characterized by cytoplasm of moderate to high electron density, well developed rough endoplasmic reticulum and nuclei with thick peripheral accumulations of heterochromatin. There were no basal lamina and caveolae along the cell membrane. It was observed that single fibroblast-like cells formed probable small gap junctions with muscle cells of both circular and longitudinal layers. Fibroblast-like cells with the same features were also observed in the region of the deep muscular plexus in both +/+ and W/W-nu mice. The present observation, together with our previous studies on rats and guinea-pigs, suggest the common presence of gap junctions or gap junction-like structures on fibroblast-like cells in the gastrointestinal musculature and their involvement in the regulatory system of gastrointestinal motility by passing electrical or molecular signals to influence the state of muscle tonus. (C) 2000 Elsevier Science B.V. All rights reserved.