Identification and expression cloning of a leptin receptor, OB-R
Identification and expression cloning of a leptin receptor, OB-R
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DOI：
10.1016/0092-8674(95)90151-5
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发表时间：
1995-12-29
期刊：
影响因子：
64.5
通讯作者：
Tepper, RI
中科院分区：
文献类型：
--
作者：
Tartaglia, LA;Dembski, M;Tepper, RI
ob基因产物瘦素是调节体重的重要循环信号。为了确定高亲和力的瘦素结合位点，我们产生了一系列的瘦素-碱性磷酸酶（AP）融合蛋白以及[I-125]瘦素。在对细胞系和组织进行结合调查后，我们确定了小鼠脉络丛中的瘦素结合位点。从小鼠脉络丛制备cDNA表达文库，并用瘦素-AP融合蛋白筛选以鉴定瘦素受体（OB-R）。OB-R是与IL-6受体、G-CSF受体和LIF受体的gp 130信号转导组分最相关的单跨膜受体。OB-R mRNA不仅在脉络丛中表达，而且在包括下丘脑在内的其他几种组织中也有表达。编码OB-R的基因的遗传作图显示，它在含有db位点的小鼠4号染色体的5.1cM间隔内。
The ob gene product, leptin, is an important circulating signal for the regulation of body weight. To identify high affinity leptin-binding sites, we generated a series of leptin-alkaline phosphatase (AP) fusion proteins as well as [I-125]leptin. After a binding survey of cell lines and tissues, we identified leptin-binding sites in the mouse choroid plexus. A cDNA expression library was prepared from mouse choroid plexus and screened with a leptin-AP fusion protein to identify a leptin receptor (OB-R). OB-R is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Genetic mapping of the gene encoding OB-R shows that it is within the 5.1 cM interval of mouse chromosome 4 that contains the db locus.