Human heme oxygenase cDNA and induction of its mRNA by hemin.
Human heme oxygenase cDNA and induction of its mRNA by hemin.
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DOI：
10.1111/j.1432-1033.1988.tb13811.x
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发表时间：
1988-02
期刊：
影响因子：
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通讯作者：
Tadashi Yoshida;P. Biró;T. Cohen;Rita K. M. Müller;S. Shibahara
中科院分区：
文献类型：
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作者：
Tadashi Yoshida;P. Biró;T. Cohen;Rita K. M. Müller;S. Shibahara
氯化高铁血红素处理可提高人巨噬细胞中血红素加氧酶的活性和mRNA水平。利用氯化高铁血红素处理的人巨噬细胞制备的富含聚(A)的RNA，我们在Okayama-Berg载体中构建了一个cDNA文库。用大鼠基因片段筛选文库，获得人血红素加氧酶基因文库，并进行核苷酸序列分析。推测的人血红素加氧酶由288个氨基酸组成，相对分子质量为32,800 Da。大鼠与人血红素加氧酶的氨基酸序列同源性为80%。和大鼠血红素加氧酶一样，人的酶在其羧基末端有一个假定的膜片段，这可能是血红素加氧酶插入内质网所必需的。大鼠和人的血红素加氧酶都没有半胱氨酸残基。最近我们发现大鼠的血红素加氧酶是一种热休克蛋白[J.Biol.化学。262,12889-12892(1987年)，因此我们研究了热处理对人巨噬细胞和胶质瘤细胞中血红素加氧酶诱导的影响。与氯化高铁血红素处理相比，热处理对两种人细胞株的血红素加氧酶活性及其mRNA水平均无明显影响。这些结果表明，人类血红素加氧酶可能不是一种热休克蛋白。
Hemin treatment increased both activity and mRNA level of heme oxygenase in human macrophages. Using poly(A)-rich RNA prepared from human macrophages treated with hemin, we have constructed a cDNA library in the Okayama-Berg vector. The human heme oxygenase cDNA was isolated by screening this library with a rat cDNA and was subjected to nucleotide sequence analysis. The deduced human heme oxygenase is composed of 288 amino acids with a molecular mass of 32,800 Da. The homology in amino acid sequences between rat and human heme oxygenase is 80%. Like rat heme oxygenase, human enzyme has a putative membrane segment at its carboxyl terminus, which is probably essential for the insertion of heme oxygenase into endoplasmic reticulum. Both rat and human heme oxygenase have no cysteine residues. Recently we have shown that rat heme oxygenase is a heat-shock protein [J. Biol. Chem. 262, 12889-12892 (1987)], and therefore we examined the effects of heat treatment on the induction of heme oxygenase in human macrophages and glioma cells. In contrast to hemin treatment, heat treatment had no apparent effects in either human cell line on the activity of heme oxygenase and its mRNA levels. These results suggest that human heme oxygenase may not be a heat-shock protein.