Jasmonate-induced Changes in Flavonoid Metabolism in Barley (Hordeum vulgare) Leaves
Jasmonate-induced Changes in Flavonoid Metabolism in Barley (Hordeum vulgare) Leaves
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茉莉酸诱导的大麦叶中类黄酮代谢的变化
DOI：
10.1271/bbb.66.2176
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
H. Iwamura
中科院分区：
文献类型：
--
作者：
A. Ishihara;Yuki Ogura;S. Tebayashi;H. Iwamura
研究了茉莉酸对大麦（Hordeum vulgare L.）次生代谢的影响。反相高效液相色谱分析显示，一种特定的化合物的量增加，在切除的大麦叶段已处理与JA。该化合物经光谱分析和碱性水解纯化，鉴定为6′′′-阿魏苷皂苷(1)。一种相关的化合物，6′′′- sinapoyl皂苷(2)，也被发现在独立于JA处理的切除叶片中积累。这些化合物的积累伴随着皂苷(3)含量的降低。[8,9- 13c]对香豆酸和[2,3,4,5,6- 2h] l -苯丙氨酸被有效地掺入到1和2中的羟基肉桂基部分，而标记的前体掺入皂苷部分的程度很小。这些结果表明，1和2的羟基肉桂基部分是通过苯丙氨酸途径由苯丙氨酸重新合成的，皂苷部分主要由3的组成池提供。
The effects of jasmonic acid (JA) on secondary metabolism in barley (Hordeum vulgare L.) were investigated. A reversed-phase HPLC analysis revealed that the amount of a particular compound increased in excised barley leaf segments that had been treated with JA. This compound was purified and identified as 6´´´-feruloylsaponarin (1) by spectroscopic analyses and alkaline hydrolysis. A related compound, 6´´´-sinapoylsaponarin (2), was also found to accumulate in excised leaves independently of the JA treatment. The accumulation of these compounds was accompanied by a decrease in the saponarin (3) content. [8,9-13C]p-Coumaric acid and [2,3,4,5,6-2H]L-phenylalanine were effectively incorporated into the hydroxycinnamoyl moieties in 1 and 2, while the degree of incorporation of the labeled precursors into the saponarin part was small. These findings indicate that the hydroxycinnamoyl moieties of 1 and 2 are synthesized de novo from phenylalanine via the phenylpropanoid pathway, and that the saponarin part is mainly provided by the constitutive pool of 3.