Spermatogenesis in agricultural workers exposed to dibromochloropropane (DBCP)
Spermatogenesis in agricultural workers exposed to dibromochloropropane (DBCP)
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接触二溴氯丙烷 (DBCP) 的农业工人的精子发生
DOI：
10.1007/bf01770028
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发表时间：
1979
影响因子：
2.7
通讯作者：
J. Eldridge
中科院分区：
文献类型：
--
作者：
S. Sandifer;R. T. Wilkins;C. Loadholt;L. G. Lane;J. Eldridge
方法 来自六个不同州的 73 名受试者参与了这项研究。在建立潜在暴露的农业工人研究小组时，我们一致尝试着眼于所有职业群体，包括配方设计师、商业施用人员、农民和农场工人、研究人员和销售人员。在选择研究候选人时，制造商代表提供了主要分销商或直接施用者的姓名，然后项目工作人员与他们联系以识别个别工人。一旦找到，纳入研究的唯一标准是工人必须使用过或可能接触过 DBCP。所有愿意参与的个人都被纳入该研究。虽然它既不可行，也无意获得所有 DBCP 用户的随机样本，但上述选择过程（在不进行选择性筛选的情况下识别尽可能多的 DBCP 用户）往往会产生一个充分代表目标人群的研究组。体检由医生完成，每个人除了提供有关其生殖和农药使用史的具体信息外，还被要求填写一份病史调查问卷。精液样本是从所有未接受输精管切除术的男性身上采集的。样本的贡献是通过自慰完成的。立即检查样品和/或准备用干冰运送到参与实验室进行分析，由同一位经验丰富的技术人员进行重新计数。检查精液的体积和精子密度t（每毫升数百万个精子）和形态。对所有参与者进行的实验室测试包括睾酮、卵泡刺激素 (FSH) 和黄体生成素 (LH) 的测量。血清 FSH、LH 和睾酮水平通过灵敏和特异的放射免疫测定法进行测量。 C
METHODSSeventy-three subjects from six different states participated in the study. In establishing the study group of potentially exposed agricultural workers, a concerted attempt was made to look at all occupational groups including formulators, commercial applicators, farmers and farm workers, researchers, and sales personnel. In selecting candidates for the study, manufacturer representatives furnished names of primary distributors or direct applicators who were then contacted by project staff for identification of individual workers. Once located, the sole criterion for inclusion in the study was that a worker must have used or have been otherwise potentially exposed to DBCP. All such individuals willing to participate were included in the study. While it was neither feasible nor was it intended to obtain a random sample of all DBCP users, the above selection process-identification of as many DBCP users as possible without selective screening-would tend to produce a study group which adequately represented the target population.Physical examinations were completed by a physician, and each individual was asked to complete a medical history questionnaire in addition to providing specific information as to his reproductive and pesticide use history. Semen samples were collected from all non-vasectomized men. Contribution of sample was accomplished through masturbation. Samples were examined immediately and/or prepared for shipment on dry ice to participating laboratories for analysis where recounts were made by the same e~ perienced technician. Semen was examined for volume and sperm density t (millions of spermatozoa per mL) and morphology. Laboratory tests on all participan~ included measurements of testosterone, follicle stimulating hormone (FSH), and luteinizing hormone (LH). Serum FSH, LH, and testosterone~ evels were measured by senstive and specific radioimmunoassays. C