Interaction of human recombination proteins Rad51 and Rad54
Interaction of human recombination proteins Rad51 and Rad54
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DOI：
10.1093/nar/25.20.4106
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发表时间：
1997-10-15
影响因子：
14.9
通讯作者：
Radding, CM
中科院分区：
文献类型：
--
作者：
Golub, EI;Kovalenko, OV;Radding, CM
克隆并在大肠杆菌中表达了与酿酒酵母重组/修复蛋白Rad54结构同源的人蛋白HsRad54 cDNA，体外和体内分析表明，HsRad54蛋白与人Rad51重组酶相互作用。这种相互作用是由HsRad54蛋白的n端结构域介导的，它可以与游离和dna结合的HsRad51蛋白相互作用。
The cDNA for human protein HsRad54, which is a structural homolog of Saccharomyces cerevisiae recombination/repair protein Rad54, was cloned and expressed in Escherichia coli, As demonstrated by analysis in vitro and in vivo, HsRad54 protein interacts with human Rad51 recombinase. The interaction is mediated by the N-terminal domain of HsRad54 protein, which interacts with both free and DNA-bound HsRad51 protein.