PLANT MICROTECHNIQUE - SOME PRINCIPLES AND NEW METHODS
PLANT MICROTECHNIQUE - SOME PRINCIPLES AND NEW METHODS
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DOI：
10.1002/j.1537-2197.1968.tb06952.x
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发表时间：
1968-01-01
影响因子：
3
通讯作者：
OBRIEN, TP
中科院分区：
文献类型：
--
作者：
FEDER, N;OBRIEN, TP
植物组织学中常用的固定剂和包埋介质会破坏或严重扭曲活植物细胞的一些容易看到的结构特征。例如，在固定在FAA（福尔马林-乙酸-乙醇混合物）中并包埋在石蜡中的植物组织的染色切片中，线粒体和细胞质的细跨液泡链通常是不可见的。然而，使用合适的固定剂和包埋介质可以保留许多结构特征。特别是在非凝固性固定剂中的固定（例如，四氧化锇、丙烯醛、戊二醛、甲醛）和使用塑料作为包埋介质，并详细描述了丙烯醛固定和甲基丙烯酸乙二醇酯聚合物包埋的方法。在以这种方式制备的广泛的植物标本中，1-3微米厚的染色切片显示出组织和细胞结构的良好保存。
Some easily seen structural features of living plant cells are destroyed or badly distorted by most of the common fixatives and embedding media used in plant histology. In stained sections of plant tissues fixed in FAA (formalin-acetic acid-alcohol mixtures) and embedded in paraffin wax, for example, mitochondria andfinetrans-vacuolar strands of cytoplasm are usually not visible. Many structural features such as these can be preserved, however, with suitable fixatives and embedding media. Specifically fixation innon-coagulant fixatives (e.g., osmium tetroxide, acrolein, glutaraldehyde, formaldehyde) and the use of plastics as embedding media was recommended, and a method of fixation in acrolein and embedding in glycol methacrylate polymer was described in detail. In a wide range of plant specimens prepared in this way, stained sections 1-3 microns thick showed excellent preservation of tissue and cell structures.