EuroTracker® dyes: design, synthesis, structure and photophysical properties of very bright europium complexes and their use in bioassays and cellular optical imaging
EuroTracker® dyes: design, synthesis, structure and photophysical properties of very bright europium complexes and their use in bioassays and cellular optical imaging
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DOI：
10.1039/c4dt02785j
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发表时间：
2015-01-01
影响因子：
4
通讯作者：
Zwier, Jurriaan M.
中科院分区：
文献类型：
--
作者：
Butler, Stephen J.;Delbianco, Martina;Zwier, Jurriaan M.
最明亮的发光铕（III）配合物的发展被跟踪，包括分析的C-3-对称的核心配合物的基础上的功能化的三氮杂环壬烷和识别最合适的强吸收的发色团。策略的配合物，包括对映体纯类似物的合成，概述和时间分辨显微镜和光谱成像的应用机会强调。实用的例子介绍，包括选择性细胞器染色细胞光学成像在65 nm的分辨率和新的生物测定使用时间分辨FRET方法的发展。
The development of the brightest luminescent europium(III) complexes is traced, including analysis of the C-3-symmetric core complex based on a functionalized triazacyclononane and identification of the most suitable strongly absorbing chromophore. Strategies for the synthesis of the complexes, including enantiopure analogues, are outlined and opportunities for applications in time-resolved microscopy and spectral imaging emphasised. Practicable examples are introduced, including selective organelle staining for cellular optical imaging at 65 nm resolution and the development of new bioassays using timeresolved FRET methods.