The expression characterization of chicken uncoupling protein gene
The expression characterization of chicken uncoupling protein gene
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DOI：
10.5713/ajas.2005.1552
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发表时间：
2005-12
期刊：
影响因子：
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通讯作者：
Jianguo Zhao;H. Li;Yu-xiang Wang;H. Meng
中科院分区：
文献类型：
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作者：
Jianguo Zhao;H. Li;Yu-xiang Wang;H. Meng
UCP是线粒体内膜转运蛋白家族的成员，存在于线粒体内膜中。它们的主要功能是通过减少线粒体氧化磷酸化产生的ATP而不是产生耗散热来增加能量消耗。它们与脂肪的代谢和能量消耗的调节有关。UCP基因可作为鸡肥度的候选基因。本研究采用RT-PCR和北方杂交方法，对鸡心、肝、脾、肺、肾、肌胃、肠、脑、胸肌和腹脂等10种组织中UCP基因的表达进行了研究。RT-PCR和北方杂交结果表明，UCP基因在胸肌中特异表达。采用RT-PCR方法检测了蛋用型和肉用型鸡在孵化期、2、4、6、8周龄胸肌中UCP基因的表达水平，结果表明，UCP基因的表达水平与品种有关。除6周龄外，各周龄蛋鸡UCP基因的表达量均高于肉鸡。肉鸡UCP基因在6周龄时表达量较高，其余周龄间差异不显著;蛋鸡UCP基因在不同周龄间表达量差异不显著。实验结果还表明，胰岛素可使UCP基因的表达水平比对照组提高40%。
The UCPs are members of the mitochondrial inner membrane transporter family, present in the mitochondrial inner membrane. Their main function is increasing the energy expenditure via diminishing the resulting production of ATP from mitochondrial oxidative phosphorylation instead of yielding dissipative heat. They are associated with the metabolism of fat and regulation of energy expenditure. The UCP gene can be viewed as the candidate gene for chicken fatness. In the present study, RT-PCR and Northern Blot methods were developed to investigate the expression of the UCP gene in ten tissues including heart, liver, spleen, lung, kidney, gizzard, intestine, brain, breast muscle and abdominal fat of chicken. The results of both RT-PCR and Northern Blot methods showed that the UCP gene expressed specific in breast muscle. The expression levels of UCP gene in breast muscles from egg-type and meat-type chickens of hatching, 2, 4, 6 and 8 wk of age were detected by RT-PCR assay and results showed that the expression levels of UCP gene were related to breeds. Expression level of UCP gene in layers was higher than that in broilers at various weeks of age except at 6 wk. The UCP gene's expression was higher at 6 wk and had no significant difference among other weeks of age in broilers; in layers the expression level of UCP gene had no significant difference among weeks of age. The experiment results also showed that insulin could increase the expression level of UCP gene by 40% compared with control group.