Effect of vacuum-assisted closure combined with open bone grafting to promote rabbit bone graft vascularization.
Effect of vacuum-assisted closure combined with open bone grafting to promote rabbit bone graft vascularization.
复制标题
真空辅助闭合的作用与开放骨移植相结合以促进兔骨移植血管形成。
DOI：
10.12659/msm.892939
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发表时间：
2015-04-27
期刊：
影响因子：
--
通讯作者：
Ping A
中科院分区：
文献类型：
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作者：
Hu C;Zhang T;Ren B;Deng Z;Cai L;Lei J;Ping A
复合骨不连合并软组织缺损是治疗的难点，闭合闭合引流联合开放植骨是目前最有效的治疗方法之一。本研究旨在初步探讨VAC联合开放植骨促进兔骨移植血管化的效果和机制，为临床工作提供理论依据。将24只新西兰白色家兔随机分为实验组和对照组。移植同种异体骨，并用缝线将其与股骨近端捆绑在一起。实验组使用VAC，而对照组使用正常伤口闭合。术后第4、8、12周分别行X线片、荧光骨标记（标记盐酸四环素和钙黄绿素）、骨痂钙含量及Western blot检测移植骨中成纤维细胞生长因子-2（FGF-2）的表达，比较骨血管化率。在手术后第4、8和12周，测试结果表明，实验组中的骨痂比对照组中的骨痂更大，含有更多的钙（p<0.05），并且以更高的水平表达FGF-2（p<0.05）。荧光骨标记显示，术后8周和12周，对照组两条荧光带之间的距离明显短于实验组。VAC联合开放植骨促进兔植骨血管化。
Patients with composite bone non-union and soft tissue defects are difficult to treat. Vacuum-assisted closure (VAC) combined with open bone grafting is one of the most effective treatments at present. The aim of the present study was to preliminarily investigate the effect and mechanism of VAC combined with open bone grafting to promote rabbit bone graft vascularization, and to propose a theoretical basis for clinical work. Twenty-four New Zealand white rabbits were randomly divided into an experimental and a control group. Allogeneic bones were grafted and banded with the proximal femur with a suture. The experimental group had VAC whereas the control group had normal wound closure. The bone vascularization rate was compared based on X-ray imaging, fluorescent bone labeling (labeled tetracycline hydrochloride and calcein), calcium content in the callus, and expression of fibroblast growth factor-2 (FGF-2) in bone allografts by Western blot analysis at the 4th, 8th, and 12th week after surgery. At the 4th, 8th, and 12th week after surgery, the results of the tests demonstrated that the callus was larger, contained more calcium (p<0.05), and expressed FGF-2 at higher levels (p<0.05) in the experimental group than in the control group. Fluorescent bone labeling showed the distance between the two fluorescent ribbons was significantly shorter in the control group than in the experimental group at the 8th and 12th week after surgery. VAC combined with open bone grafting promoted rabbit bone graft vascularization.