Haemagglutination activity of plaque-forming bacteria.
Haemagglutination activity of plaque-forming bacteria.
复制标题
噬菌斑形成细菌的血凝活性。
作者：
G. Rölla;M. Kilian
牙菌斑的形成是由细菌吸附到后天形成的牙釉质膜上引起的，已知牙釉质膜含有血型物质。在本研究中，使用人红细胞作为噬菌斑细菌和细菌产物的靶标，观察到血凝。蔗糖生长，但不是葡萄糖生长的变形链球菌菌株引起血凝。与此相反，血链球菌、缓和链球菌和粘性放线菌的菌株凝集红细胞，而与培养基中的蔗糖无关。从链球菌培养物中制备的可溶性和不溶性多糖。变形杆菌引起血凝，而线性的商业多糖是无效的。总菌斑从蔗糖喂养的猴子，以及高比例的细菌菌株吸附到光滑的牙齿表面的猴子在3小时内，引起血凝。确定这些噬斑形成微生物的血凝能力的因素进行了讨论。口腔微生物对红细胞的吸附可以构成一个模型系统，从该模型系统中可以获得与初始细菌对牙齿的吸附有关的数据。
Dental plaque formation is initiated by adsorption of bacteria to the acquired enamel pellicle, which is known to contain blood group substances. In the present study, human red blood cells were used as targets for plaque bacteria and bacterial products and haemagglutination was observed. Sucrose-grown, but not glucose-grown Streptococcus mutans strains caused haemagglutination. In contrast, strains of Streptococcus sanguis, Streptococcus mitior and Actinomyces viscosus agglutinated red cells irrespectively of sucrose in the medium. Soluble and insoluble polysaccharides prepared from cultures of Strep. mutans caused haemagglutination, whereas linear, commercial polysaccharides were ineffective. Total plaque from sucrose-fed monkeys, as well as a high proportion of bacterial strains adsorbed to smooth tooth surfaces of monkeys during a 3-hour period, caused haemagglutination. The factors determining the haemagglutinating ability of these plaque-forming microorganisms are discussed. Adsorption of oral microorganisms to red blood cells may constitute a model system from which data related to initial bacterial adsorption to teeth can be obtained.