Nanostructured Surface Functionalization of Polyacrylamide Hydrogels Below the Length Scale of Hydrogel Heterogeneity
Nanostructured Surface Functionalization of Polyacrylamide Hydrogels Below the Length Scale of Hydrogel Heterogeneity
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DOI：
10.1021/acsami.2c12034
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发表时间：
2022-09-14
影响因子：
9.5
通讯作者：
Claridge, Shelley A.
中科院分区：
文献类型：
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作者：
Arango, Juan C.;Williams, Laura O.;Claridge, Shelley A.
水凝胶广泛用于聚合物材料必须与生物体接触的应用中。水凝胶网络是无定形的，在长达数百纳米的长度尺度上具有显著的异质性，在某些情况下，这对将受益于与生物分子的高度结构化相互作用的应用提出了挑战。在这里，我们表明，它是可能的，以产生有序的图案的功能团在聚丙烯酰胺水凝胶表面。我们证明，当线性模式的胺转移到聚丙烯酰胺，他们的图案与DNA在界面上的相互作用，在色谱应用中的预浓缩的潜在重要性的能力，以及为开发纳米结构的混合材料和支持细胞培养。
Hydrogels are broadly used in applications where polymer materials must interface with biology. The hydrogel network is amorphous, with substantial heterogeneity on length scales up to hundreds of nanometers, in some cases raising challenges for applications that would benefit from highly structured interactions with biomolecules. Here, we show that it is possible to generate ordered patterns of functional groups on polyacrylamide hydrogel surfaces. We demonstrate that, when linear patterns of amines are transferred to polyacrylamide, they pattern interactions with DNA at the interface, a capability of potential importance for preconcentration in chromatographic applications, as well as for the development of nanostructured hybrid materials and supports for cell culture.