A METHODICAL STUDY OF SHAPE CHANGES IN HUMAN ORAL CELLS PERTURBED BY A SIMULATED ORTHODONTIC STRAIN IN-VITRO
A METHODICAL STUDY OF SHAPE CHANGES IN HUMAN ORAL CELLS PERTURBED BY A SIMULATED ORTHODONTIC STRAIN IN-VITRO
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DOI：
10.1016/0003-9969(95)00044-p
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发表时间：
1995-09-01
影响因子：
3
通讯作者：
MELSEN, B
中科院分区：
文献类型：
--
作者：
NORTON, LA;ANDERSEN, KL;MELSEN, B
众所周知，细胞会根据物理和化学变化而改变形状。施加在牙齿上的机械载荷产生细胞扰动，导致正畸运动。建立了一个体外模型来模拟正畸运动的体内应变。校正后的力应用于人牙周韧带细胞和颊粘膜成纤维细胞（对照组）。采用双轴应变产生装置对生长在柔性聚氟乙烯膜上的重要细胞进行拉伸。此外，一种新的细胞粘合剂，cell Tak(TM)，被用来研究粘合剂底物对细胞对两种已知负荷的反应的影响。采用延时遥视显微镜观察非应变（对照）和应变细胞的形状变化，并记录细胞面积和形状随时间变化的图。梭状细胞在长达1400秒的特定时间内变得更圆。反应似乎是独立的细胞类型，应变采用，和存在的细胞粘合剂。扫描电子显微镜显示，无论细胞类型如何，与表面相对光滑的对照组相比，应激细胞表面产生了大量的微绒毛。
Cells are known to alter their shape as a response to physical and chemical changes. Mechanical loads applied to teeth produce cellular perturbations resulting in orthodontic movement. An in vitro model was developed to simulate the in vivo strain of orthodontic movement. Calibrated forces were applied to human periodontal ligament cells and buccal mucosal fibroblasts (controls). A biaxial strain-producing device was used to stretch vital cells grown on flexible polyletrafluorethylene membranes. In addition, a new cell adhesive, Cell Tak(TM), was employed to examine the effect of an adhesive substrate on the cellular response to two known loads. The shape changes of unstrained (control) and strained cells were evaluated by time-lapse telemicroscopy, and plots of time-dependent alterations in area and shape were recorded. The fusiform cells became more rounded over a given time of up to 1400 s. The responses appeared to be independent of cell type, the strain employed, and the presence of cell adhesive. Scanning electron microscopy demonstrated, irrespective of cell type, that the surface of stressed cells produced a striking number of microvilli as compared with the relatively smooth-surfaced controls.