Synergistic activation by recombinant mouse interferon-gamma and muramyl dipeptide of tumoricidal properties in mouse macrophages.
Synergistic activation by recombinant mouse interferon-gamma and muramyl dipeptide of tumoricidal properties in mouse macrophages.
复制标题
重组小鼠干扰素-γ和胞壁酰二肽协同激活小鼠巨噬细胞的抗肿瘤特性。
DOI：
10.4049/jimmunol.135.1.684
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发表时间：
1985
影响因子：
4.4
通讯作者：
I. Fidler
中科院分区：
文献类型：
--
作者：
I. Saiki;I. Fidler
小鼠腹腔巨噬细胞被激活，对B16-BL6黑色素瘤细胞具有细胞毒性，通过亚阈值的小鼠干扰素- γ （ifn - γ， 0.1至10 U/ml）和n-乙酰muramyl- l- alanyl- d -异谷氨酰胺（MDP， 0.001至10微克/ml）联合使用，但不通过pH - 2处理的ifn - γ和MDP，热处理的ifn - γ和MDP，或ifn - γ和MDP的无活性立体异构体n-乙酰muramyl- d -alanyl- d -异谷氨酰胺（MDP）联合使用。将完整的ifn - γ和MDP包封在同一脂质体中对巨噬细胞活化具有协同作用。相反，在不同的脂质体制剂中呈现相同浓度的ifn - γ和MDP不会激活巨噬细胞。这些数据使我们得出结论，将基因工程的ifn - γ和合成的MDP包封在同一个脂质体中，可以高效地在小鼠巨噬细胞中产生杀肿瘤特性的协同激活。
Mouse peritoneal macrophages were activated to become cytotoxic against B16-BL6 melanoma cells by the combination of subthreshold amounts of murine interferon-gamma (IFN-gamma; 0.1 to 10 U/ml) and N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP; 0.001 to 10 micrograms/ml), but not by the combination of pH 2-treated IFN-gamma and MDP, heat-treated IFN-gamma and MDP, or IFN-gamma and the inactive stereoisomer of MDP, N-acetyl-muramyl-D-alanyl-D-isoglutamine (MDP-D). The encapsulation of intact IFN-gamma and MDP within the same liposome preparation was synergistic for macrophage activation. In contrast, the presentation of identical concentrations of IFN-gamma and MDP in separate liposome preparations did not activate macrophages. These data allow us to conclude that the encapsulation of genetically engineered IFN-gamma and synthetically produced MDP within the same liposome is highly efficient in producing synergistic activation of tumoricidal properties in mouse macrophages.