THE MEASUREMENT OF EXTRACELLULAR WATER VOLUMES IN TISSUES BY GADOLINIUM MODIFICATION OF PROTON NMR SPIN LATTICE T-1 RELAXATION
THE MEASUREMENT OF EXTRACELLULAR WATER VOLUMES IN TISSUES BY GADOLINIUM MODIFICATION OF PROTON NMR SPIN LATTICE T-1 RELAXATION
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DOI：
10.1016/0730-725x(86)91038-6
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发表时间：
1986-01-01
影响因子：
2.5
通讯作者：
FURMANSKI P
中科院分区：
文献类型：
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作者：
BRAUNSCHWEIGER P G;SCHIFFER L;FURMANSKI P
本研究在RIF-1、M5076和Panc 02小鼠肿瘤模型中进行，以比较通过51 Cr-EDTA稀释技术和利用钆-DTPA-二甲基葡糖胺（Gd-DTPA-dimeg）对1H-NMR质子弛豫的浓度依赖性修饰的新方法在血浆和组织中进行的细胞外水测量。静脉注射0.1 ml 100 mM Gd-DTPA-dimeg后，在不同时间间隔测定组织和血浆中T1修饰的时间依赖性变化，并从小鼠血浆Gd浓度依赖性T1修饰的标准曲线中确定Gd浓度。组织和血浆Gd浓度的比较允许计算Gd-DTPA-dimeg分布体积。在未受干扰的RIF-1，M5076和Panc 02肿瘤，Gd-DTPA-dimeg的分布体积确定的T1修改技术是类似的细胞外水空间确定的51铬-EDTA稀释测定。在小鼠肝脏中，由于探针的内化，51 Cr-EDTA测定导致人为的高细胞外水空间估计值;用153 Gd-DTPA-dimeg和T1修饰法测定的肝脏中Gd-DTPA-dimeg分布体积约为150 μ l/gram。Gd-DTPA-dimeg T1修饰法还提供了RIF中细胞外水体积变化的良好近似值。地塞米松和环磷酰胺治疗后1例肿瘤。这些结果表明，Gd-DTPA-dimeg修饰的质子弛豫可能是非常有用的监测肿瘤水动力学在癌症治疗过程中的变化。
The present studies were conducted in RIF-1, M5076 and Panc02 murine tumor models to compare extracellular water measurements made by 51Cr-EDTA dilution techniques and a new method which exploits the concentration dependent modification of 1H-NMR proton relaxation by Gadolinium-DTPA-dimethyl glucamine (Gd-DTPA-dimeg) in plasma and tissues. The time dependent changes in T1 modification in tissue and plasma were determined at various intervals after i.v. injection of 0.1 ml of 100 mM Gd-DTPA-dimeg and Gd concentrations deterimined from standard curves of Gd concentration dependent T1 modification of mouse plasma. Comparison of tissue and plasma Gd concentrations permitted the calculation of Gd-DTPA-dimeg distribution volumes. In unperturbed RIF-1, M5076 and Panc02 tumors, Gd-DTPA-dimeg distribution volumes determined by the T1 modification technique were similar to extracellular water spaces determined by 51Cr-EDTA dilution assays. In mouse liver, the 51Cr-EDTA assay resulted in artifactually high extracellular water space estimates due to internalization of the probe; Gd-DTPA-dimeg distribution volumes determined in liver with both the 153Gd-DTPA-dimeg and by the T1 modification method were approximately 150 ul/gram. The Gd-DTPA-dimeg T1 modification method also provided good approximations of changes in extracellular water volumes in RIF-1 tumors after dexamethasone and cyclophosphamide treatments. These results indicate that Gd-DTPA-dimeg modification of proton relaxation may be extremely useful for monitoring changes in tumor water dynamics during cancer therapy.