Investigation of volatile biomarkers in lung cancer blood using solid-phase microextraction and capillary gas chromatography-mass spectrometry
Investigation of volatile biomarkers in lung cancer blood using solid-phase microextraction and capillary gas chromatography-mass spectrometry
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DOI：
10.1016/j.jchromb.2004.05.015
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发表时间：
2004-09-05
影响因子：
3
通讯作者：
Li, N
中科院分区：
文献类型：
--
作者：
Deng, CH;Zhang, XM;Li, N
本工作建立了固相微萃取(SPME)和气相色谱-质谱仪(GC-MS)相结合的方法，用于肺癌挥发性生物标志物的研究。对顶空固相微萃取条件(纤维涂层、萃取温度和萃取时间)和解吸条件进行了优化，并将其应用于人血中挥发性物质的测定。为了寻找肺癌的生物标志物，用本方法对肺癌血和对照血中的挥发性化合物进行了调查。肺癌患者血中正己醛和正庚醛的浓度明显高于对照血。己醛和庚醛两个分子被认为是肺癌的生物标志物。通过呼气和血液中挥发物的比较，证明呼气中的己醛和庚醛来源于血液，呼气分析筛查肺癌是可行的。这些结果表明，SPME/GC-MS是一种简便、快速、灵敏的方法，非常适合于人血中挥发性疾病标志物的研究。(C)2004爱思唯尔B.V.保留所有权利。
In the present work, solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) was developed for investigation of lung cancer volatile biomarkers. Headspace SPME conditions (fiber coating, extraction temperature and extraction time) and desorption conditions were optimized and applied to determination of volatiles in human blood. To find the biomarkers, of lung cancer, investigation of volatile compounds in lung cancer blood and control was performed by using the present method. Concentrations of hexanal and heptanal in lung cancer blood were found to be much higher than those in control blood. The two molecules of hexanal and heptanal were regarded as biomarkers of lung cancer. By comparison of volatiles in breath and in blood, it is demonstrated that hexanal and heptanal in breath were originated from blood and screening of lung cancer by breath analysis be feasible. These results show that SPME/GC-MS is a simple, rapid and sensitive method very suitable for investigation of volatile disease markers in human blood. (C) 2004 Elsevier B.V. All rights reserved.