Enhanced degradation of softwood versus hardwood by the white-rot fungus Pycnoporus coccineus.
Enhanced degradation of softwood versus hardwood by the white-rot fungus Pycnoporus coccineus.
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DOI：
10.1186/s13068-015-0407-8
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发表时间：
2015
影响因子：
6.3
通讯作者：
Rosso MN
中科院分区：
文献类型：
--
作者：
Couturier M;Navarro D;Chevret D;Henrissat B;Piumi F;Ruiz-Dueñas FJ;Martinez AT;Grigoriev IV;Riley R;Lipzen A;Berrin JG;Master ER;Rosso MN
白腐担子菌真菌是植物生物质的强效降解者，具有使所有木质纤维素成分矿化的能力。近期的比较基因组学研究表明，这些真菌使用多种酶来降解木材。然而，为了理解导致木质纤维素分解的酶促机制，还需要更深入的功能分析。多孔菌属真菌朱红密孔菌BRFM310在针叶木和阔叶木上均生长良好。在本研究中，我们分析了该真菌对软木（松树）和硬木（山杨）原料的早期反应，并测试了分泌的酶对木质纤维素解构的影响。
转录组学和蛋白质组学分析表明，分别在松树和山杨上生长的朱红密孔菌显示出相似的与木质素和多糖降解有关的转录本和酶集合。特别是，以木质素为靶向的氧化还原酶（如锰过氧化物酶）的表达在两种木材上培养时均增加。在松树和山杨上生长过程中分泌的酶集合在从松树中释放糖类方面比从山杨中更有效，并且对残留固体的表征揭示了松树和山杨纤维表面的多糖转化。
对可溶性糖和固体残留物的综合分析表明朱红密孔菌分泌的酶适合用于软木降解。对酶处理木材样品的溶解产物和残留表面化学性质的分析指出了不同的朱红密孔菌分泌蛋白组在纤维渗透方面的差异。因此，除了在朱红密孔菌基因组、转录组和分泌组中鉴定出的多种碳水化合物活性酶（CAZymes）之外，我们还讨论了几个参数，如锰过氧化物酶的丰度以及细胞色素P450和果胶降解在真菌对软木转化功效方面的潜在作用。
本文的在线版本（doi:10.1186/s13068 - 015 - 0407 - 8）包含补充材料，可供授权用户使用。
White-rot basidiomycete fungi are potent degraders of plant biomass, with the ability to mineralize all lignocellulose components. Recent comparative genomics studies showed that these fungi use a wide diversity of enzymes for wood degradation. Deeper functional analyses are however necessary to understand the enzymatic mechanisms leading to lignocellulose breakdown. The Polyporale fungus Pycnoporus coccineus BRFM310 grows well on both coniferous and deciduous wood. In the present study, we analyzed the early response of the fungus to softwood (pine) and hardwood (aspen) feedstocks and tested the effect of the secreted enzymes on lignocellulose deconstruction. Transcriptomic and proteomic analyses revealed that P. coccineus grown separately on pine and aspen displayed similar sets of transcripts and enzymes implicated in lignin and polysaccharide degradation. In particular, the expression of lignin-targeting oxidoreductases, such as manganese peroxidases, increased upon cultivation on both woods. The sets of enzymes secreted during growth on both pine and aspen were more efficient in saccharide release from pine than from aspen, and characterization of the residual solids revealed polysaccharide conversion on both pine and aspen fiber surfaces. The combined analysis of soluble sugars and solid residues showed the suitability of P. coccineus secreted enzymes for softwood degradation. Analyses of solubilized products and residual surface chemistries of enzyme-treated wood samples pointed to differences in fiber penetration by different P. coccineus secretomes. Accordingly, beyond the variety of CAZymes identified in P. coccineus genome, transcriptome and secretome, we discuss several parameters such as the abundance of manganese peroxidases and the potential role of cytochrome P450s and pectin degradation on the efficacy of fungi for softwood conversion. The online version of this article (doi:10.1186/s13068-015-0407-8) contains supplementary material, which is available to authorized users.