Passive transfer of virus-specific antibodies confers protection against reproductive failure induced by a virulent strain of porcine reproductive and respiratory syndrome virus and establishes sterilizing immunity
Passive transfer of virus-specific antibodies confers protection against reproductive failure induced by a virulent strain of porcine reproductive and respiratory syndrome virus and establishes sterilizing immunity
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DOI：
10.1006/viro.2002.1612
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发表时间：
2002-10-10
期刊：
影响因子：
3.7
通讯作者：
Laegreid, WW
中科院分区：
文献类型：
--
作者：
Osorio, FA;Galeota, JA;Laegreid, WW
介导针对猪繁殖与呼吸综合征病毒（PRRSV）的保护性免疫的免疫机制还不清楚。PRRSV特异性体液免疫应答被认为是无效的，并且可能对宿主有害。通过在妊娠猪中的被动转移实验，检测了PRRSV抗体在抗该病毒的高度流产株感染的保护性免疫中的功能。接受来自PRRSV恢复期、超免疫动物的PRRSV免疫球蛋白（IG）的一组妊娠后备母猪（n = 6）的全部被完全保护免于生殖失败，如通过断奶时（15日龄）后代的95%存活率所判断的。另一方面，在接受抗伪狂犬病病毒（PRV）IG的匹配对照组中，所有动物均表现出明显的生殖失败，断奶时存活率为4%。除了保护妊娠雌性动物免受临床生殖疾病外，PRRSV IG的被动转移还防止了攻击病毒感染母鼠，并阻止了其垂直传播，如母鼠组织中完全不存在传染性PRRSV及其后代中不存在感染所证明的。总之，这些结果表明PRRSV-Ig能够赋予针对PRRSV的保护性免疫，此外这些Ig可以提供体内杀菌免疫。(C)2002 Elsevier Science（美国）。
Immune mechanisms mediating protective immunity against porcine reproductive and respiratory syndrome virus (PRRSV) are not well understood. The PRRSV-specific humoral immune response has been dismissed as being ineffective and perhaps deleterious for the host. The function of PRRSV antibodies in protective immunity against infection with a highly abortifacient strain of this virus was examined by passive transfer experiments in pregnant swine. All of a group of pregnant gilts (n = 6) that received PRRSV immunoglobulin (Ig) from PRRSV-convalescent, hyperimmune animals were fully protected from reproductive failure as judged by 95% viability of offspring at weaning (15 days of age). On the other hand, the totality of animals in a matched control group In = 6) receiving anti-pseudorabies virus (PRV) Ig exhibited marked reproductive failure with 4% survival at weaning. Besides protecting the pregnant females from clinical reproductive disease, the passive transfer of PRRSV Ig prevented the challenge virus from infecting the dams and precluded its vertical transmission, as evidenced by the complete absence of infectious PRRSV from the tissues of the dams and lack of infection in their offspring. In summary, these results indicate that PRRSV-Igs are capable of conferring protective immunity against PRRSV and furthermore that these Igs can provide sterilizing immunity in vivo. (C) 2002 Elsevier Science (USA).