Comparative in vitro activities of posaconazole, voriconazole, itraconazole, and amphotericin B against Aspergillus and Rhizopus, and synergy testing for Rhizopus
Comparative in vitro activities of posaconazole, voriconazole, itraconazole, and amphotericin B against Aspergillus and Rhizopus, and synergy testing for Rhizopus
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DOI：
10.1080/13693780801975576
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发表时间：
2008-01-01
期刊：
影响因子：
2.9
通讯作者：
Mcnicholas, Paul
中科院分区：
文献类型：
--
作者：
Arikan, Sevtap;Sancak, Banu;Mcnicholas, Paul
我们比较了泊沙康唑、伏立康唑、伊曲康唑和阿替霉素B对曲霉菌临床分离株的体外活性。和根霉属，并探讨了泊沙康唑和阿替霉素B对根霉的体外相互作用。82株曲霉菌临床分离株。(43烟曲霉29 A. flavus，7 A.尼日尔，2 A. terreus，1 A.根据CLSI M38-A微量稀释指南测试了11株根霉分离株。泊沙康唑对曲霉菌的体外抗菌活性。还用Etest进行了研究。泊沙康唑和阿替霉素B联合应用对红曲霉的毒力较低。采用棋盘格法对分离株进行了研究。伏立康唑是体外抗曲霉菌活性最强的药物，其次是泊沙康唑、伊曲康唑和阿替霉素B，活性依次降低。在与R.在分离株中，泊沙康唑是最有效的药物，其次是伊曲康唑和阿替霉素B。伏立康唑对根霉没有明显的抑制作用。泊沙康唑Etest对曲霉菌属的MIC（g/ml）发现其显著低于CLSI微量稀释法（24和48 h时分别比CLSI MIC低4-9和3-7倍）。泊沙康唑和阿替霉素B之间的相互作用对所有R.检测了100株分离株;重要的是，未观察到拮抗作用。
We compared the in vitro activities of posaconazole, voriconazole, itraconazole, and amphotericin B against clinical isolates of Aspergillus spp. and Rhizopus spp., and explored the in vitro interaction between posaconazole and amphotericin B against Rhizopus spp. Clinical strains of 82 Aspergillus spp. (43 Aspergillus fumigatus, 29 A. flavus, 7 A. niger, 2 A. terreus, 1 A. nidulans) and 11 Rhizopus oryzae isolates were tested in accordance with CLSI M38-A microdilution guidelines. In vitro activity of posaconazole against Aspergillus spp. was also investigated with the Etest. The combination of posaconazole and amphotericin B against R. oryzae isolates was investigated by the checkerboard methodology. Voriconazole was the most active drug in vitro against Aspergillus spp., followed by posaconazole, itraconazole, and amphotericin B, in order of decreasing activity. In studies with R. oryzae isolates, posaconazole was found to be the most potent drug followed by itraconazole and amphotericin B. Voriconazole had no meaningful activity against Rhizopus. Posaconazole Etest MICs (g/ml) with Aspergillus spp. were found to be considerably lower than those obtained with the CLSI microdilution method (4-9 and 3-7 two-fold lower than CLSI MICs at 24 and 48 h, respectively). The interaction between posaconazole and amphotericin B was indifferent for all R. oryzae isolates tested; importantly no antagonism was observed.