Paracrine endocrinology of cardiac telocytes in culture
Paracrine endocrinology of cardiac telocytes in culture
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DOI：
10.4103/2470-7511.248218
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发表时间：
2017-05
期刊：
影响因子：
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通讯作者：
Hua Li;Youen Zhang;Wenbin Zhang;Xin Zhong;Gang Zhao;J. Ge
中科院分区：
文献类型：
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作者：
Hua Li;Youen Zhang;Wenbin Zhang;Xin Zhong;Gang Zhao;J. Ge
目的：本研究旨在表征培养物中心肌细胞（TC）群体的旁分泌细胞因子，并研究其调节心脏稳态的机制。方法和结果：我们通过活细胞成像记录了具有典型形态的心脏TC的体外进化，重点是释放的podomer、podom和细胞外囊泡。通过 Quantibody 试剂盒对心脏 TC 的旁分泌细胞因子进行定量，重点关注信号转导、细胞增殖、凋亡和发育等生物过程。总的来说，这些发现表明，心脏 TC 通过激活细胞外信号调节激酶/Jun N 末端激酶信号通路，通过一组细胞因子的旁分泌作用协调心脏微血管内皮细胞 (EC) 的增殖。结论：心脏 TC 通过特定细胞因子的旁分泌活性调节心脏基本生理并维持微血管 EC 增殖。
Objective: The study aimed to characterize the paracrine cytokines of cardiac telocyte (TC) populations in culture and to investigate their mechanisms in regulating cardiac homeostasis. Methods and Results: We recorded the in vitro evolution of cardiac TCs with typical morphology through live cell imaging, with emphasis on released podomers, podoms, and extracellular vesicles. Paracrine cytokines of the cardiac TCs were quantified by the Quantibody kit, with the focus being on of biological processes such as signal transduction, cell proliferation, apoptosis, and development. The findings in aggregate illustrated that cardiac TCs orchestrated the proliferation of cardiac microvascular endothelial cells (ECs) through paracrine action of a panel of cytokines through activation of the extracellular signal-regulated kinase/Jun N-terminal kinase signaling pathway. Conclusion: Cardiac TCs modulated cardiac essential physiology and maintained microvascular EC proliferation through paracrine activity of defined cytokines.