Mollicellins O-R, Four New Depsidones Isolated from the Endophytic Fungus Chaetomium sp. Eef-10
Mollicellins O-R, Four New Depsidones Isolated from the Endophytic Fungus Chaetomium sp. Eef-10
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Mollicellins OâR，从内生真菌毛壳菌属中分离出的四种新的缩氨酸。
DOI：
10.3390/molecules23123218
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发表时间：
2018-12-01
期刊：
影响因子：
4.6
通讯作者：
Shan, Tijiang
中科院分区：
文献类型：
--
作者：
Ouyang, Jinkui;Mao, Ziling;Shan, Tijiang
从分离自桉树（Eucalyptus exserta）的内生真菌毛壳菌（Chaetomium sp. Eef-10）的培养物中分离得到四个新的缩酚酮化合物mollicellins O-R（化合物1-4），沿着三个已知化合物5-7。新化合物的结构通过1D和2D NMR和HR-ESI-MS谱进行了鉴定。通过将已知化合物的光谱数据与公布的值进行比较来鉴定它们。评价化合物1-7对金黄色葡萄球菌（敏感和抗性菌株）、大肠杆菌、根癌土壤杆菌、鼠伤寒沙门氏菌、流泪假单胞菌、青枯雷尔氏菌、水疱黄单胞菌的抗菌活性和对两种人类癌细胞系（HepG 2和Hela）的细胞毒性活性。Mollicellin H（6）对S. aureus ATCC 29213对金黄色葡萄球菌的抑制率为6.21 μ g/mL。金黄色葡萄球菌N50、耐甲氧西林金黄色葡萄球菌（MRSA）。Mollicellin O（1）和Mollicellin I（7）对S. aureus ATCC 29213和S.金黄色葡萄球菌N50。Mollicellin G（5）对两种人癌细胞系都有活性，IC 50值为19.64和13.97 μ g/mL，而化合物6和7仅对一种细胞系显示出细胞毒性活性。此外，mollicellin O（1）显示出基于DPPH自由基清除的抗氧化活性，IC 50值为71.92 μ g/mL。
Four new depsidones, mollicellins O-R (compounds 1-4), along with three known compounds 5-7, were isolated from cultures of the fungus Chaetomium sp. Eef-10, an endophyte isolated from Eucalyptus exserta. The structures of the new compounds were elucidated by analysis of the 1D and 2D NMR and HR-ESI-MS spectra. The known compounds were identified by comparison of their spectral data with published values. Compounds 1-7 were evaluated for antibacterial activities against Staphylococcus aureus (sensitive and resistant strains), Escherichia coli, Agrobacterium tumefaciens, Salmonella typhimurium, Pseudomonas lachrymans, Ralstonia solanacearum, Xanthomonas vesicatoria and cytotoxic activities against two human cancer cell lines (HepG2 and Hela). Mollicellin H (6) displayed best antibacterial activity, with IC50 values of 5.14 mu g/mL against S. aureus ATCC29213 and 6.21 mu g/mL against S. aureus N50, MRSA, respectively. Mollicellin O (1) and mollicellin I (7) also exhibited antibacterial activities against S. aureus ATCC29213 and S. aureus N50. Mollicellin G (5) was active against both two human cancer cell lines, with IC50 values of 19.64 and 13.97 mu g/mL while compounds 6 and 7 only showed cytotoxic activity against one cell line. In addition, mollicellin O (1) showed antioxidant activity based on DPPH radical scavenging, with an IC50 value of 71.92 mu g/mL.