Antibacterial and antibiofilm activity of bone marrow-derived human mesenchymal stem cells secretome against Vibrio cholerae
Antibacterial and antibiofilm activity of bone marrow-derived human mesenchymal stem cells secretome against Vibrio cholerae
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DOI：
10.1016/j.micpath.2019.103867
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发表时间：
2020-02-01
影响因子：
3.8
通讯作者：
Najar-peerayeh, Shahin
中科院分区：
文献类型：
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作者：
Bahroudi, Mahboube;Bakhshi, Bita;Najar-peerayeh, Shahin
霍乱弧菌在水生环境中生存和传播的能力，加上有效抗微生物药物的稀缺，特别是那些对多重耐药菌株有效的药物，突出了治疗霍乱弧菌感染的替代非抗生素方法的必要性。本研究的目的是专门研究未经刺激的间充质干细胞分泌组对霍乱弧菌杀伤和生物膜形成的潜在直接影响，霍乱弧菌是一种非侵入性肠道细菌病原体的代表。bmMSCs的特征是存在CD44和CD73，不存在CD45和CD34分子标记。此外，通过免疫组织学（IHC）方法评估MSCs向脂肪细胞和成骨细胞谱系的自我再生和分化能力。在肉汤微稀释实验中，未受刺激的MSCs上清液对霍乱弧菌的抑菌活性从10(8)CFU/ml降至10(7)CFU/ml，并在1:8 ~ 1:8 8的稀释倍数下呈剂量依赖性（P < 0.05）。通过平板结晶紫法评估了未经预处理的间充质干细胞分泌组在预防生物膜形成中的作用，并显示出对霍乱弧菌的高抗生物膜活性，且呈剂量依赖性。由于间充质干细胞分泌组的抑菌机制不同于传统抗生素，加上其抗菌膜活性，提出了其作为对抗多重耐药病原体的新型治疗方法的应用，而不必担心产生耐药性。
The ability of V. cholerae to survive and spread in the aquatic environment combined with the scarcity of effective antimicrobial agents, especially those effective against multidrug-resistant strains highlights the need for alternative non-antibiotic approaches for the treatment of V. cholerae infections. The aim of this study was to specifically examine the potential direct effect of unstimulated MSC secretome on V. cholerae killing and biofilm formation as a representative of non-invasive enteric bacterial pathogen. The bmMSCs were characterized by the presence of CD44 and CD73 and the absence of CD45 and CD34 molecular markers. Moreover, self-regeneration and differentiation capacity of MSCs into adipocytes and osteogenic lineages was assessed by immunohistology (IHC) method. The antibacterial activity of unstimulated MSCs supernatant against V. cholerae in broth micro-dilution assay decreased the bacterial suspension from 10(8) CFU/ml to 10(7) CFU/ml and showed a significant antimicrobial activity in a dose-dependent manner at dilutions of 1:8 to 1:128 (P < 0.05). The role of MSC secretome without preconditioning in the prevention of biofilm formation was assessed through plate-crystal violet assay and showed high antibiofilm activity against V. cholerae also in dose-dependent manner. As antibacterial mechanisms of MSC secretome are different from conventional antibiotics, together with its antibiofilm activity, proposes its application as a novel therapeutic approach combatting multi-drug resistant pathogens with no fear of developing antimicrobial resistance.