Single-cell analysis of Drosophila larval neuromuscular synapses
Single-cell analysis of Drosophila larval neuromuscular synapses
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DOI：
10.1006/dbio.2000.9983
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发表时间：
2001-01-01
影响因子：
2.7
通讯作者：
Chiba, A
中科院分区：
文献类型：
--
作者：
Hoang, B;Chiba, A
果蝇的神经肌肉系统已被广泛应用于突触发育的研究。在胚胎中，这个模型系统的细胞组件被很好地建立起来，唯一识别的运动神经元显示出与不同肌肉的特定连接。这些知识对于分析轴突引导和单细胞分辨率的突触匹配机制是必不可少的。相比之下，到目前为止，幼虫神经肌肉突触的细胞身份还很难确定。目前尚不清楚在胚胎中看到的突触连接是否持续存在，也不知道单个运动终末如何在幼虫阶段分化。在这项研究中，我们结合单个突触环的单细胞染料标记和整个神经系统的反染色来表征来自四个神经分支(ISN、SNA、SNB和SND)的30个运动神经元轴突的突触伙伴和突触分化。我们还显示了4个幼虫运动神经元(Rp3、Rp5、V和MN13-Ib)的胞体位置和它们所发育的神经类型。我们的观察结果支持以下结论：(1)一块肌肉只有1个运动神经元轴突，而4个不同类型的运动神经元轴突可以支配同一肌肉。(2)各运动神经元轴突形成的轴突类型可能受细胞自主因素的影响。这些数据为以高细胞分辨率研究突触分化、维持和可塑性的特性提供了基础。(C)2001年学术出版社。
The neuromuscular system of Drosophila has been widely used in studies on synaptic development. In the embryo, the cellular components of this model system are well established, with uniquely identified motoneurons displaying specific connectivity with distinct muscles. Such knowledge is essential to analyzing axon guidance and synaptic matching mechanisms with single-cell resolution. In contrast, to date the cellular identities of the larval neuromuscular synapses are hardly established. It is not known whether synaptic connections seen in the embryo persist, nor is it known how individual motor endings may differentiate through the larval stages. In this study, we combine single-cell dye labeling of individual synaptic boutons and counterstaining of the entire nervous system to characterize the synaptic partners and bouton differentiation of the 30 motoneuron axons from four nerve branches (ISN, SNa, SNb, and SNd). We also show the cell body locations of 4 larval motoneurons (RP3, RP5, V, and MN13-Ib) and the types of innervation they develop. Our observations support the following: (1) Only 1 motoneuron axon of a given bouton type innervates a single muscle, while up to 4 motoneuron axons of different bouton types can innervate the same muscle. (2) The type of boutons which each motoneuron axon forms is likely influenced by cell-autonomous factors. The data offer a basis for studying the properties of synaptic differentiation, maintenance, and plasticity with a high cellular resolution. (C) 2001 Academic Press.