Studies on the chemical constituents and anticancer activity of Saxifraga stolonifera (L) Meeb
Studies on the chemical constituents and anticancer activity of Saxifraga stolonifera (L) Meeb
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DOI：
10.1016/j.bmc.2007.10.072
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发表时间：
2008-02-01
影响因子：
3.5
通讯作者：
Zhou, Xia
中科院分区：
文献类型：
--
作者：
Chen, Zhuo;Liu, Yu-Mei;Zhou, Xia
虎耳草是一种常绿双子叶植物，因其抗癌活性而被确定为重要的中药资源。在本报告中，对贵州省发现的S. stolonifera (L) Meeb 的化学成分进行了调查。从 S. stolonifera 植物的乙醇提取物中分离出 10 种化合物，并鉴定为 n-C-31 H-64 (1)、(n-C17H35)(2)CO (2)、β-谷甾醇 (3)、n-C29H60 (4)、岩白菜素 (5)、原儿茶酸 (6)、没食子酸 (7)、槲皮素 3-O-α-L-鼠李糖苷 (8)、槲皮素 (9) 和槲皮素 3-O-β-D-吡喃葡萄糖苷 (10)。其中n-C31H64 (1)、(n-C17H35)(2)CO (2)、β-谷甾醇(3)、n-C29H60 (4)为首次从该植物中分离得到。采用MTT法、显微镜观察、DNA片段化、流式细胞仪分析等方法评价匍匐花成分对人胃癌细胞系BGC-823的抗癌活性。结果发现，槲皮素暴露 72 It 后可抑制细胞活力。此外，DNA 梯分析表明，槲皮素可以以浓度和时间依赖性方式诱导 DNA 链断裂。流式细胞术分析显示，槲皮素在48 h内可诱导11.82%的BGC-823细胞凋亡。这些数据表明槲皮素是一种能够诱导 BGC-823 细胞凋亡的潜在药物。 (C) 2007 Elsevier Ltd. 保留所有权利。
Saxifraga stolonifera, an evergreen dicotyledon, has been identified as an important resource in Chinese medicine due to its anticancer activity. In the present report, chemical components of S. stolonifera (L) Meeb which is found in Guizhou province were investigated. Ten compounds were isolated from ethanol extracts of S. stolonifera plant and were identified as n-C-31 H-64 (1), (n-C17H35)(2)CO (2), beta-sitosterol (3), n-C29H60 (4), Bergenin (5), Protocatechuic acid (6), Gallic acid (7), Quercitrin 3-O-alpha-L-rhamnoside (8), Quercetin (9), and Quercetin 3-O-beta-D-glucopyranoside (10). Among them, n-C31H64 (1), (n-C17H35)(2)CO (2), beta-sitosterol (3), and n-C29H60 (4) were isolated from this plant for the first time. The anticancer activities of S. stolonifera constituents on human gastric carcinoma cell line BGC-823 were evaluated by MTT assay and microscopic observation, DNA fragmentation, and flow cytometry analysis assay. It was found that quercetin could inhibit cell viability after 72 It of exposure. Furthermore, DNA ladder assay revealed that quercetin could induce DNA strand break in a concentration- and time-dependent fashion. Flow cytometric analysis shows that quercetin can induce 11.82% BGC-823 cell apoptosis in 48 h. These data reveal that quercetin is a potential agent capable of inducing apoptosis in BGC-823 cells. (C) 2007 Elsevier Ltd. All rights reserved.