Dietary Cocoa Powder Improves Hyperlipidemia and Reduces Atherosclerosis in apoE Deficient Mice through the Inhibition of Hepatic Endoplasmic Reticulum Stress.
Dietary Cocoa Powder Improves Hyperlipidemia and Reduces Atherosclerosis in apoE Deficient Mice through the Inhibition of Hepatic Endoplasmic Reticulum Stress.
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DOI：
10.1155/2016/1937572
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发表时间：
2016
影响因子：
4.6
通讯作者：
Liu E
中科院分区：
文献类型：
--
作者：
Guan H;Lin Y;Bai L;An Y;Shang J;Wang Z;Zhao S;Fan J;Liu E
可可粉富含类黄酮，对人体健康有许多有益作用，包括抗氧化和抗炎作用。我们研究的目的是调查可可粉的摄入是否对高脂血症和动脉粥样硬化有影响，并检查潜在的分子机制。我们给 apoE 基因敲除小鼠喂食添加 0.2%（低组）或 2%（高组）可可粉的西方饮食，持续 12 周。与对照组相比，食用可可粉的组的血浆胆固醇水平和主动脉粥样硬化显着降低。对主动脉粥样硬化病变的 mRNA 谱分析显示，与对照组相比，可可粉组中与凋亡、脂质代谢和炎症相关的多个基因的表达显着降低，而抗凋亡基因 Bcl2 显着增加。 RT-PCR 分析和蛋白质印迹表明，含有可可粉的饮食可抑制肝内质网应激的表达。这些数据表明，摄入可可粉可以改善高脂血症和动脉粥样硬化，这种有益作用可能是通过抑制肝内质网应激来介导的。
Cocoa powder is rich in flavonoids, which have many beneficial effects on human health, including antioxidative and anti-inflammatory effects. The aim of our study was to investigate whether the intake of cocoa powder has any influence on hyperlipidemia and atherosclerosis and examine the underlying molecular mechanisms. We fed apoE knockout mice a Western diet supplemented with either 0.2% (low group) or 2% (high group) cocoa powder for 12 weeks. The groups fed dietary cocoa powder showed a significant reduction in both plasma cholesterol levels and aortic atherosclerosis compared to the control group. Analysis of mRNA profiling of aortic atherosclerotic lesions revealed that the expression of several genes related to apoptosis, lipid metabolism, and inflammation was significantly reduced, while the antiapoptotic gene Bcl2 was significantly increased in the cocoa powder group compared to the control. RT-PCR analysis along with Western blotting revealed that a diet containing cocoa powder inhibited the expression of hepatic endoplasmic reticulum stress. These data suggest that cocoa powder intake improves hyperlipidemia and atherosclerosis, and such beneficial effects are possibly mediated through the suppression of hepatic endoplasmic reticulum stress.