MONOCLONAL-ANTIBODIES FOR IDENTIFICATION OF BORRELIA-AFZELII SP-NOV - ASSOCIATED WITH LATE CUTANEOUS MANIFESTATIONS OF LYME BORRELIOSIS
MONOCLONAL-ANTIBODIES FOR IDENTIFICATION OF BORRELIA-AFZELII SP-NOV - ASSOCIATED WITH LATE CUTANEOUS MANIFESTATIONS OF LYME BORRELIOSIS
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DOI：
10.3109/00365549309008525
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发表时间：
1993-01-01
影响因子：
--
通讯作者：
POSTIC, D
中科院分区：
文献类型：
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作者：
CANICA, MM;NATO, F;POSTIC, D
根据DNA同源性将莱姆疏螺旋体分离株分为3个基因种：伯氏疏螺旋体、加里尼伯氏疏螺旋体和VS461群。用单抗、H3TS和D6分别鉴定了伯氏伯氏杆菌和加里尼伯氏杆菌，但没有单抗鉴定VS461群。从属于VS461群的菌株中分离到两株能与OSPB和OspA蛋白反应的单抗，分别为I17.3和J8.3，应命名为B.afzelii sp.11月24株细菌被鉴定为阿氏假单胞菌。11月，从皮损中分离到11株，慢性萎缩性肢端皮炎(ACA)6株，慢性迁徙红斑(ECM)5株。虽然ACA在美国鲜为人知，但在北欧经常有报道，那里有B.afzelii sp.11月通常是孤立的。本研究记录了B.afzelii sp.的参与。11月作为ACA的一种特殊病原体。
Borrelia isolates associated with Lyme borreliosis were previously divided into 3 genospecies, B. burgdorferi sensu stricto, B. garinii and group VS461, on the basis of DNA homology. B. burgdorferi sensu stricto and B. garinii were identified by monoclonal antibodies (MAbs), H3TS and D6 respectively, but no MAbs were available to identify group VS461. Two MAbs were produced, I 17.3 and J 8.3 which reacted with OspB and OspA proteins, respectively, of strains belonging to group VS461, which should be named B. afzelii sp. nov. 24 strains were assigned to B. afzelii sp. nov., 11 of them being isolated from skin lesions, 6 from acrodermatitis chronica atrophicans (ACA) and 5 from erythema chronicum migrans (ECM). Although quite unknown in the USA, ACA has frequently been reported in northern Europe where B. afzelii sp. nov. is commonly isolated. This study documents the involvement of B. afzelii sp. nov. as a specific aetiological agent of ACA.