The specialized mitotic behavior of human embryonic stem cells
The specialized mitotic behavior of human embryonic stem cells
复制标题
人类胚胎干细胞的特殊有丝分裂行为
DOI：
10.1007/s00441-021-03544-2
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发表时间：
2021-11-03
影响因子：
3.6
通讯作者：
Li，Dengwen
中科院分区：
文献类型：
--
作者：
Lyu，Rui;Wu，Xuemei;Li，Dengwen
人胚胎干细胞（human embryonic stem cells，hESC）是一种具有自我更新能力的多能细胞，来源于囊胚内细胞团。有丝分裂是生物体生存和繁殖的基础，并负责将复制的染色体平均分配到子细胞中。有丝分裂功能障碍与多种人类疾病有关，尤其是癌症。hESC具有独特的细胞周期分布，但目前尚不清楚hESC的有丝分裂活性与其增殖和分化的确切关系。在此，我们通过慢病毒感染建立了稳定表达GFP-α-tubulin和mCherry-H2 B的hESC细胞系，以详细分析和可视化有丝分裂。在中期，有丝分裂纺锤体更小，更宽，含有更大比例的星形微管比正常细胞。此外，纺锤体微管更稳定，染色体排列在hESC中比体细胞更快。我们还发现纺锤体组装检查点在hESC中是功能性的。因此，这些发现揭示了一个专门的有丝分裂行为的hESC。
Human embryonic stem cells (hESCs) are self-renewing and pluripotent cells that originate from the inner cell mass of the blastocyst. Mitosis is fundamental to organism survival and reproduction and is responsible for the equal distribution of duplicated chromosomes into daughter cells. Mitotic dysfunction is associated with a wide variety of human diseases, not least cancer. hESCs have a unique cell cycle distribution, but it is unclear exactly how the mitotic activity of hESCs is related to their proliferation and differentiation. Here, we established a cell line of hESCs stably expressing GFP-α-tubulin and mCherry-H2B by lentiviral infection to analyze and visualize mitosis in detail. During metaphase, the mitotic spindle was smaller and wider and contained a greater proportion of astral microtubules than normal cells. In addition, spindle microtubules were more stable, and chromosome alignment was faster in hESCs than in somatic cells. We also found that the spindle assembly checkpoint was functional in hESCs. These findings thus reveal a specialized mitotic behavior of hESCs.