Adenomatous polyposis coli (APC) protein expression in primary and metastatic serous ovarian carcinoma
Adenomatous polyposis coli (APC) protein expression in primary and metastatic serous ovarian carcinoma
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DOI：
10.1177/106689690201000302
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发表时间：
2002-07-01
影响因子：
1.2
通讯作者：
Nesland, JM
中科院分区：
文献类型：
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作者：
Karbova, E;Davidson, B;Nesland, JM
本研究的目的是探讨原发性和转移性浆液性卵巢癌中腺瘤性结肠息肉病（APC）的蛋白表达。另外分析β-连环蛋白和E-钙粘蛋白的表达。对113个原发性（n=56）和转移性（n=57）病变进行APC、E-钙粘蛋白和β-连环蛋白的免疫组织化学染色。对染色程度进行评分。对原发和转移部位免疫反应性的可能差异以及分析的蛋白质之间的相关性进行了统计学评价。在67/113（59%）肿瘤中发现APC的胞浆免疫反应性，最常见于大多数（>50%）细胞中。在102/113（90%）的癌中检测到E-钙粘蛋白，而在109/113（97%）的标本中检测到β-连环蛋白。在3/113（3%）例标本中观察到β-连环蛋白的核表达，均为APC阴性。APC和β-连环蛋白通常共表达，但这一发现未能达到统计学显著性（p=0.11）。在E-钙粘蛋白和β-连环蛋白表达之间观察到显著关联（p=0.001）。APC在原发灶和转移灶中的表达相似（p>0.05）。总之，APC表达是缺乏在相当数量的原发性和转移性卵巢癌，但这一发现是很少耦合到β-连环蛋白的核积累。这些发现支持β-连环蛋白信号通过Wingless/Wnt通路在卵巢癌中的作用。APC在浆液性卵巢癌中表达下调的机制及其意义尚不清楚。
The aim of this study was to investigate protein expression of adenomatous polyposis coli (APC) in primary and metastatic serous ovarian carcinoma. The expression of beta-catenin and E-cadherin was additionally analyzed. one hundred and thirteen primary (n=56) and metastatic (n=57) lesions were immunohistochemically stained for APC, E-cadherin, and beta-catenin. Staining extent was scored. Possible differences in immunoreactivity in primary and metastatic sites and the association between the proteins analyzed were evaluated statistically. Cytoplasmic immunoreactivity for APC was found in 67/113 (59%) tumors, most often in the majority (>50%) of cells. E-cadherin was detected in 102/113 (90%) carcinomas, while beta-catenin was expressed in 109/113 (97%) specimens. Nuclear expression of beta-catenin was seen in 3/113 (3%) specimens, all negative for APC. APC and beta-catenin were often coexpressed, but this finding failed to reach statistical significance (p=0.11). A significant association was seen between E-cadherin and beta-catenin expression (p=0.001). APC expression was comparable in primary and metastatic tumors (p>0.05). In conclusion, APC expression is absent in a considerable number of both primary and metastatic ovarian carcinomas, but this finding is only rarely coupled to nuclear accumulation of beta-catenin. These findings support the role for beta-catenin signaling via the Wingless/Wnt pathway in ovarian carcinoma. The mechanism behind the down-regulated expression of APC in serous ovarian carcinoma and its significance has yet to be elucidated.