A piglet model for detection of hypoxic-ischemic brain injury with magnetic resonance imaging.
A piglet model for detection of hypoxic-ischemic brain injury with magnetic resonance imaging.
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DOI：
10.1080/02841850802334224
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发表时间：
2008-11
期刊：
影响因子：
--
通讯作者：
Hald JK
中科院分区：
文献类型：
--
作者：
Munkeby BH;De Lange C;Emblem KE;Bjørnerud A;Kro GA;Andresen J;Winther-Larssen EH;Løberg EM;Hald JK
Munkeby BH，De Lange C，Emblem Ke，Bj？rnerud A，Kro GAB，Anresen J，Winther-Larssen EH，L？磁共振成像检测缺氧缺血性脑损伤仔猪模型的建立。《无线电学报》2008；49：1049-1057。早期发现窒息新生儿的缺氧缺血性损伤是很重要的，因为目前的预后因素是不充分的。此外，如果及时开始，治疗性干预可能会带来额外的好处。目的：评估常规磁共振成像(MRI)、扩散加权成像(DWI)、扩散张量成像(DTI)和质子磁共振波谱(MRS)等综合检查方法能否在缺氧缺血后7小时发现仔猪模型的病理改变。将10头仔猪置于低氧30分钟后，用21%氧气复氧7小时。分别于HI前和HI后7h行1.5T磁共振成像。进行单体素质子磁共振成像，测量基底节的表观扩散系数(ADC)和分数各向异性(FA)。MRS鉴定出N-乙酰天冬氨酸(NAA)、胆碱(Cho)、肌酸(Cr)和乳酸(Lac)。实验结束后行组织学和微管相关蛋白2(MAP-2)染色。与基线相比，7h后ADC值、NAA/Cho、NAA/Cr值均显著降低(P<0.001)，FA值显著升高(P<0.025)。7h后Lac/Cho和Lac/NAA比值较基础值显著升高(P<；0.001)。坏死的存在与ADC降低(RS=0.91)和Lac的存在(RS=0.80)有很好的相关性。组织学和MAP-2染色显示，8头仔猪出现90%以上的坏死，1头出现60%以上的坏死，1头无坏死。磁共振弥散成像和质子磁共振波谱可以检测到仔猪缺氧7h后脑内的HI损伤。弥散加权成像和磁共振波谱可提供有用的预后信息。这种仔猪模型有可能用于模拟临床情况，并适合于进一步研究HI损伤。
Munkeby BH, de Lange C, Emblem KE, Bjørnerud A, Kro GAB, Andresen J, Winther-Larssen EH, Løberg EM, Hald JK. A piglet model for detection of hypoxic-ischemic brain injury with magnetic resonance imaging. Acta Radiol 2008;49:1049–1057. Early detection of hypoxic-ischemic (HI) injury in the asphyxic newborn is important because present prognostic factors are inadequate. Furthermore, therapeutic interventions may have additional benefit if initiated in time. To assess whether the use of a combined protocol including conventional magnetic resonance imaging (MRI), diffusion-weighted imaging (DWI), diffusion tensor imaging (DTI), and proton MR spectroscopy (MRS) could detect pathological findings in a piglet model 7 hours after HI. Ten piglets were submitted to HI for 30 min followed by reoxygenation with 21% O2 for 7 hours. MRI at 1.5T was done prior to and 7 hours after the HI. Single-voxel proton MRS was performed, and apparent diffusion coefficient (ADC) and fractional anisotropy (FA) were measured in the basal ganglia. MRS identified N-acetylaspartate (NAA), choline (Cho), creatine (Cr), and lactate (Lac). Histology and microtubule-associated protein 2 (MAP-2) staining was performed in the basal ganglia at the end of the experiment. Compared to baseline, ADC, NAA/Cho, and NAA/Cr were significantly reduced after 7 hours (P < 0.001, P = 00.01, and P = 00.05, respectively) and FA values were increased (P <0.025). The ratios of Lac/Cho and Lac/NAA were significantly higher after 7 hours compared to baseline (P <0.001). Presence of necrosis correlated well with reduced ADC (RS = 0.91) and presence of Lac (RS = 0.80). Histology and MAP-2 staining showed more than 90% necrosis in eight piglets, 60% in one piglet, and no necrosis in one piglet. Diffusion MRI and proton MRS can detect HI injury in the piglet brain 7 hours after hypoxia. DWI and MRS can be used to give useful prognostic information. This piglet model may potentially be used to mimic clinical situations and is suitable for further research investigating HI injury.
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3.6
作者：
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