TaSRG, a wheat transcription factor, significantly affects salt tolerance in transgenic rice and Arabidopsis
TaSRG, a wheat transcription factor, significantly affects salt tolerance in transgenic rice and Arabidopsis
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TaSRG 是一种小麦转录因子，显着影响转基因水稻和拟南芥的耐盐性
DOI：
10.1016/j.febslet.2011.03.055
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发表时间：
2011-04-20
期刊：
影响因子：
3.5
通讯作者：
Huang, Zhanjing
中科院分区：
文献类型：
--
作者：
He, Xiaoliang;Hou, Xiaona;Huang, Zhanjing
根据我们对小麦耐盐突变体RH8706-49的基因芯片分析结果，我们发现并克隆了一个含有DUF662保守结构域的未知盐诱导基因，我们将该基因命名为TaSRG(Triticum Aestivum Salt Response Gene)。实时荧光定量聚合酶链式反应分析表明，该基因的表达受盐、干旱、寒冷、ABA等胁迫条件的影响。与野生型植物相比，TaSRG在野生型拟南芥中的过表达提高了植物的耐盐性。此外，亚细胞定位显示TaSRG编码一种主要在细胞核中发现的蛋白质。我们的结果表明，小麦TaSRG可能编码一种转录因子，可用于提高广泛植物的逆境耐受性。(C)2011年欧洲生化学会联合会。爱思唯尔出版公司版权所有。
Based on our microarray-analysis results of the salt tolerant wheat mutant, RH8706-49, an unknown salt-induced gene containing a conserved DUF662 domain was identified and cloned; we named this gene TaSRG (Triticum aestivum Salt Response Gene). Real-time quantitative PCR analyses showed that the expression of this gene was affected by salt, drought, cold, ABA and other stress conditions. The overexpression of TaSRG in wild-type Arabidopsis resulted in increased salt tolerance compared with wild-type plants. In addition, subcellular localisation revealed that TaSRG encodes a protein that was found mainly in the nucleus. Our results suggest that wheat TaSRG might encode a transcription factor that could be utilised for enhancing stress tolerance in a wide range of plants. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.