Enhanced myocardial cathepsin B expression in patients with dilated cardiomyopathy
Enhanced myocardial cathepsin B expression in patients with dilated cardiomyopathy
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DOI：
10.1016/j.ejheart.2005.09.004
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发表时间：
2006-05-01
影响因子：
18.2
通讯作者：
Yingzhen Yang
中科院分区：
文献类型：
--
作者：
Junbo Ge;Gang Zhao;Yingzhen Yang
目的：组织蛋白酶B是一种重要的溶酶体蛋白酶，在心肌梗死中参与细胞凋亡和肌原纤维蛋白降解。本研究的目的是研究衰竭和非衰竭人类心脏中心肌组织蛋白酶B的表达。方法：从20名扩张型心肌病患者和5名因技术原因无法移植的非衰竭供体心脏的移植左心室中采集组织样本。采用免疫组化、逆转录-聚合酶链反应（RT-PCR）和Western blotting检测心肌组织蛋白酶B的表达。结果：组织蛋白酶B在衰竭和非衰竭心脏中均呈阳性表达。组织蛋白酶B在mRNA和蛋白水平的表达在衰竭心脏中显著高于非衰竭心脏。相关性分析显示，心力衰竭患者组织蛋白酶B mRNA和蛋白水平与EF呈负相关（分别为r = 0.66，p = 0.002和r = 0.492，p = 0.028）。结论：心力衰竭患者心肌组织蛋白酶B表达增加，提示组织蛋白酶B可能参与心力衰竭的发生、发展。(c)2005年欧洲心脏病学会。Elsevier B. V.出版，保留所有权利。
Objective: Cathepsin B is a prominent lysosomal protease and is involved in apoptosis as well as degradation of myofibrillar proteins in myocardial infarction. The aim of this study was to investigate myocardial cathepsin B expression in failing and non-failing human hearts.Methods: Tissue samples were taken from transplanted left ventricles from 20 patients with dilated cardiomyopathy and 5 non-failing donor hearts that could not be transplanted for technical reasons. Myocardial cathepsin B expression was determined by immunohistochemistry, the reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. Apoptosis was assessed by TUNEL staining.Results: Positive cathepsin B staining was found in failing and non-failing hearts. The expression of cathepsin B at mRNA and protein levels was significantly higher in failing hearts compared with non-failing hearts. Correlation analysis revealed that cathepsin B at mRNA and protein levels negatively correlated with EF (r = 0.66, p = 0.002 and r = 0.492, p = 0.028, respectively) in patients with heart failure. The apoptotic index was 0.015 +/- 0.006 in failing hearts and 0.002 +/- 0.001 in non-failing hearts (p < 0.01).Conclusion: Increased myocardial expression of cathepsin B was found in patients with heart failure suggesting that cathepsin B might play a role in the genesis and development of heart failure. (c) 2005 European Society of Cardiology. Published by Elsevier B.V. All rights reserved.