Taxol, an anticancer drug produced by an endophytic fungus Bartalinia robillardoides Tassi, isolated from a medicinal plant, Aegle marmelos Correa ex Roxb.
Taxol, an anticancer drug produced by an endophytic fungus Bartalinia robillardoides Tassi, isolated from a medicinal plant, Aegle marmelos Correa ex Roxb.
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DOI：
10.1007/s11274-007-9530-4
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发表时间：
2008-05-01
影响因子：
4.1
通讯作者：
Muthumary, J.
中科院分区：
文献类型：
--
作者：
Gangadevi, V.;Muthumary, J.
紫杉醇是一种重要的抗肿瘤药物，临床应用广泛。从药用植物菠萝中分离到一株内生真菌Bartalinia robillardoides（AMB-9），并对其进行了产紫杉醇的筛选。根据真菌培养物的形态和孢子的特征对真菌进行了鉴定。该真菌在MID液体培养基中生长，并通过色谱法和光谱法分析紫杉醇的存在。通过HPLC定量该内生真菌产生的紫杉醇的量。该菌株的紫杉醇产量为187.6 μ g/L，表明该菌株可作为一种潜在的基因工程材料，以提高紫杉醇的产量。从该真菌培养物的有机提取物中分离得到的真菌紫杉醇在体外对BT 220、H116、Int 407、HL 251和HLK 210人癌细胞具有很强的细胞毒活性。
Taxol is an important anticancer drug widely used in the clinic. An endophytic fungus Bartalinia robillardoides (strain AMB-9) was isolated from Aegle marmelos, a medicinal plant and screened for taxol production. The fungus was identified based on the morphology of the fungal culture and the characteristics of the spores. This fungus was grown in MID liquid medium and analyzed chromatographically and spectrometrically, for the presence of Taxol. The amount of taxol produced by this endophytic fungus was quantified by HPLC. It produced 187.6 mu g/L of taxol which suggests that the fungus can serve as a potential material for genetic engineering to improve the production of Taxol. This fungal taxol isolated from the organic extract of this fungal culture, has strong cytotoxic activity towards BT 220, H116, Int 407, HL 251 and HLK 210 human cancer cells in vitro, tested by Apoptotic assay.